Rabbit ADAMDEC1 Polyclonal Antibody | anti-ADAMDEC1 antibody

ADAMDEC1 Polyclonal Antibody

Cat. Num. AAA3007231

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADAMDEC1; Polyclonal Antibody; ADAMDEC1 Polyclonal Antibody; ADAM DEC1 (EC: 3.4.24.-); A disintegrin and metalloproteinase domain-like protein decysin-1; ADAM-like protein decysin-1; anti-ADAMDEC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADAMDEC1 polyclonal antibody detects endogenous levels of ADAMDEC1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 391

• Applicable Applications for anti-ADAMDEC1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to residues in Human ADAMDEC1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADAMDEC1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate Lane3:H9C2 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADAMDEC1 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasm and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ADAMDEC1 antibody

ADAMDEC1 (ADAM-like, decysin 1), also known as decysin, is a 470 amino acid secreted protein that belongs to the disintegrin metalloproteinase family. Expressed primarily in dendritic cells (DCs) of the small intestine, spleen and lymph nodes, ADAMDEC1 can bind one zinc ion per subunit and is thought to be involved in controlling the immune response. ADAMDEC1 expression is induced in maturing DC cells in response to T-cell signals and its expression is upegulated during differentiation of primary monocytes into macrophages. ADAMDEC1 contains one peptidase M12B domain, a prematurely terminated disintegrin domain and, unlike other ADAM family members, does not have an intracellular tail or a cysteine-rich domain.

NCBI and Uniprot Product Information

• NCBI GI #: 223671930
• NCBI Accession #: NP_001138743.1
• NCBI GenBank Nucleotide #: NP_001138743.1
• UniProt Accession #: O15204
• Molecular Weight: ~ 53kDa
• NCBI Official Full Name: ADAM DEC1 isoform 2
• UniProt Protein Name: ADAM DEC1
• Protein Family: ADAM
• UniProt Gene Name: ADAMDEC1
• UniProt Synonym Gene Names: ADAM-like protein decysin-1
• UniProt Entry Name: ADEC1_HUMAN

Uniprot Description

Function: May play an important role in the control of the immune response and during pregnancy

By similarity.

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Subcellular location: Secreted

By similarity.

Tissue specificity: Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in monocyte and highly expressed in macrophage. Absent in immature DC. Ref.1 Ref.5

Induction: Induced during DC maturation and up-regulated in response to T-cell signals. In macrophage up-regulated by bacterial lipopolysaccharides (LPS). Up-regulated by 1-alpha,25-dihydroxyvitamin D3 during differentiation of primary monocyte into macrophage. Ref.1 Ref.5

Sequence similarities: Contains 1 disintegrin domain.Contains 1 peptidase M12B domain.