Western Blot (WB)
(Western blot analysis of EPHX2 expression in MCF7 (A), Jurkat (B) whole cell lysates.)
Rabbit EPHX2 Polyclonal Antibody | anti-EPHX2 antibody
Anti-EPHX2 Antibody
Western Blot (WB)
(Western blot analysis of EPHX2 expression in MCF7 (A), Jurkat (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EPHX2 expression in MCF7 (A), Jurkat (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EPHX2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EPHX2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]
Uniprot Description
EPHX2: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
Protein type: EC 3.3.2.10; Lipid Metabolism - arachidonic acid; Hydrolase; EC 3.1.3.76
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: cytoplasm; cytosol; peroxisome
Molecular Function: epoxide hydrolase activity; lipid phosphatase activity; lipid-phosphate phosphatase activity; magnesium ion binding; phosphoric monoester hydrolase activity; protein homodimerization activity; receptor binding; toxin binding
Biological Process: cellular calcium ion homeostasis; cholesterol homeostasis; dephosphorylation; drug metabolic process; epoxygenase P450 pathway; inflammatory response; phospholipid dephosphorylation; positive regulation of vasodilation; regulation of blood pressure; response to toxin; stilbene catabolic process; xenobiotic metabolic process
Disease: Hypercholesterolemia, Familial
Research Articles on EPHX2
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Product Notes
The EPHX2 ephx2 (Catalog #AAA8245110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EPHX2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EPHX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/1000; IHC: 1/50 - 1/100. Researchers should empirically determine the suitability of the EPHX2 ephx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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