Rabbit anti-Human EPGN Polyclonal Antibody | anti-EPGN antibody

EPGN, NT (EPGN, Epigen, Epithelial mitogen) (MaxLight 550)

Cat. Num. AAA6295948

• Gene Names: EPGN; EPG; PRO9904; ALGV3072
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EPGN; Polyclonal Antibody; NT (EPGN; Epigen; Epithelial mitogen) (MaxLight 550); Epithelial mitogen; anti-EPGN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EPGN.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-EPGN antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-51 in the N-terminal region of human EPGN.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of EPGN (arrow) in 293 cell lysates (2ug/lane) using MBS6009572 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EPGN gene.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS6009572 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EPGN antibody

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.

Product Categories/Family for anti-EPGN antibody

Antibodies; Proteins

References

1. Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008). 2. Kochupurakkal, B.S., et al. J. Biol. Chem. 280(9):8503-8512(2005). 3. Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004). 4. Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). 5. Strachan, L., et al. J. Biol. Chem. 276(21):18265-18271(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 61806622
• NCBI GeneID: 255324
• NCBI Accession #: NP_001013460.1
• NCBI GenBank Nucleotide #: NM_001013442.1
• UniProt Accession #: Q6UW88; A1BMM3; A1BMM4; A1BMM5; A1BMM6; A1BMM7; A1BMM8; A8K090
• Molecular Weight: 7.9 kDa
• NCBI Official Synonym Full Names: epithelial mitogen
• NCBI Official Symbol: EPGN
• NCBI Official Synonym Symbols: EPG; PRO9904; ALGV3072
• NCBI Protein Information: epigen
• UniProt Protein Name: Epigen
• Protein Family: Epigen
• UniProt Gene Name: EPGN
• UniProt Synonym Gene Names: EPG
• UniProt Entry Name: EPGN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. This protein has been reported to have high mitogenic activity but low affinity for its receptor. Expression of this transcript and protein have been reported in cancer specimens of the breast, bladder, and prostate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

Epigen: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: growth factor activity; epidermal growth factor receptor binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; positive regulation of cell proliferation; MAPKKK cascade; angiogenesis; positive regulation of epithelial cell proliferation