Sheep anti-Human EOMES Polyclonal Antibody | anti-EOMES antibody

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2)

Cat. Num. AAA627230

• Gene Names: EOMES; TBR2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: EOMES; Polyclonal Antibody; EOMES (Eomesodermin Homolog; T-box Brain Protein 2; T-brain-2; TBR-2; TBR2); Anti -EOMES (Eomesodermin Homolog; anti-EOMES antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EOMES. Species sequence homology: mouse (91%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.

• Applicable Applications for anti-EOMES antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot. Other applicationb s not tested.; Dilution: Western Blot: 1ug/ml
• Immunogen: Purified, E. coli-derived recombinant human EOMES (aa471-686; Accession # O95936).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of EOMES: 15ug of whole cell extracts BGOIV (-) or BGOIV mesoendoderm differentiated (+) were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with 1g/ml sheep anti-EOMES antibody.)

Related Product Information for anti-EOMES antibody

EOMES (Eomesodermin; Eo from Greek meaning "dawn"/early in mesoderm; also TBR2) is a 72kD member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686aa in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T-box (aa276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa471-686, human EOMES shares 91% aa identity with mouse EOMES.

Product Categories/Family for anti-EOMES antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 22538470
• NCBI GeneID: 8320
• NCBI Accession #: NP_005433.2
• NCBI GenBank Nucleotide #: NM_005442.3
• UniProt Accession #: O95936; Q8TAZ2; Q9UPM7; B7Z4I2
• Molecular Weight: 72,732 Da
• NCBI Official Full Name: eomesodermin homolog isoform 2
• NCBI Official Synonym Full Names: eomesodermin
• NCBI Official Symbol: EOMES
• NCBI Official Synonym Symbols: TBR2
• NCBI Protein Information: eomesodermin homolog; T-box brain protein 2
• UniProt Protein Name: Eomesodermin homolog
• Protein Family: Eomesodermin
• UniProt Gene Name: EOMES
• UniProt Synonym Gene Names: TBR2; T-brain-2; TBR-2
• UniProt Entry Name: EOMES_HUMAN

NCBI Description

This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

EOMES: Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Up-regulated in CD8+ T-cells simultaneously stimulated with TGFB1 and IL4/interleukin-4. Expressed in CD8+ T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 3p24.1

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription, DNA-dependent; CD8-positive, alpha-beta T cell differentiation during immune response; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; olfactory bulb development; endodermal cell fate specification; stem cell maintenance; cerebral cortex regionalization; negative regulation of transcription from RNA polymerase II promoter; cerebral cortex neuron differentiation; trophectodermal cell differentiation; cardioblast differentiation; mesoderm formation; endoderm formation; interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; brain development; positive regulation of cell differentiation