Western Blot (WB)
(AARS2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AARS2 antibody detected the AARS2 protein (arrow).)
Rabbit anti-Human tRNA Synthetase, Alanyl Polyclonal Antibody
tRNA Synthetase, Alanyl, ID (AARS2, Probable Alanyl-tRNA Synthetase, Mitochondrial, Alanine-tRNA Ligase, Putative, Alanyl-tRNA Synthetase-like, AARSL, KIAA1270) (HRP)
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
tRNA Synthetase; Alanyl; Polyclonal Antibody; ID (AARS2; Probable Alanyl-tRNA Synthetase; Mitochondrial; Alanine-tRNA Ligase; Putative; Alanyl-tRNA Synthetase-like; AARSL; KIAA1270) (HRP); anti-tRNA Synthetase; Alanyl antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AARS2.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
985
Applicable Applications for anti-tRNA Synthetase, Alanyl antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:10-1:50
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa373-402 from the center region of human AARS2.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Western Blot (WB)
(AARS2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AARS2 antibody detected the AARS2 protein (arrow).)
Western Blot (WB)
(AARS2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AARS2 antibody detected the AARS2 protein (arrow).)
Immunohistochemistry (IHC)
(AARS2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AARS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(AARS2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AARS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(AARS2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(AARS2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-tRNA Synthetase, Alanyl antibody
References
1. Bonnefond, L., Biochemistry 44(12), 4805-4816 (2005). 2. Nakayama, M., Genome Res. 12(11), 1773-1784 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
107.326
NCBI Official Full Name
alanine--tRNA ligase, mitochondrial
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Product Notes
The tRNA Synthetase, Alanyl (Catalog #AAA6503712) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The tRNA Synthetase, Alanyl, ID (AARS2, Probable Alanyl-tRNA Synthetase, Mitochondrial, Alanine-tRNA Ligase, Putative, Alanyl-tRNA Synthetase-like, AARSL, KIAA1270) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's tRNA Synthetase, Alanyl can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:10-1:50 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the tRNA Synthetase, Alanyl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "tRNA Synthetase, Alanyl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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