Immunohistochemistry (IHC)
(MBS9609128 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse ENTK Polyclonal Antibody | anti-ENTK antibody
ENTK Antibody
Gene Names
TMPRSS15; ENTK; PRSS7
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ENTK; Polyclonal Antibody; ENTK Antibody; PRSS 7; PRSS7; Enterokinase; Enterokinase catalytic subunit; Enteropeptidase; Enteropeptidase catalytic light chain; Enteropeptidase non catalytic heavy chain; ENTK_HUMAN; PROENTEROKINASE; protease; serine; 7 (enterokinase); Serine protease 7; TMPRSS15; Transmembrane protease serine 15; anti-ENTK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ENTK antibody detects endogenous levels of total ENTK
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1019
Applicable Applications for anti-ENTK antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human ENTK
Subcellular Location
Membrane.
Tissue Specificity
Intestinal brush border.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (86%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9609128 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609128 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of ENTK using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of ENTK using Jurkat whole cell lysates)
Related Product Information for anti-ENTK antibody
Function: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
Subunit Structure: Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.
Post-translational Modifications: The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
Similarity: Belongs to the peptidase S1 family.
Subunit Structure: Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.
Post-translational Modifications: The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
Similarity: Belongs to the peptidase S1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 112 kDa
Predicted: 113 kDa
Predicted: 113 kDa
NCBI Official Full Name
enteropeptidase
NCBI Official Synonym Full Names
transmembrane serine protease 15
NCBI Official Symbol
TMPRSS15
NCBI Official Synonym Symbols
ENTK; PRSS7
NCBI Protein Information
enteropeptidase
UniProt Protein Name
Enteropeptidase
UniProt Gene Name
TMPRSS15
UniProt Synonym Gene Names
ENTK; PRSS7
NCBI Description
This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]
Uniprot Description
Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
Research Articles on ENTK
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Product Notes
The ENTK tmprss15 (Catalog #AAA9609128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENTK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENTK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the ENTK tmprss15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENTK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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