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Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. brain section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Rabbit ENT1 Polyclonal Antibody | anti-SLC29A1 antibody

ENT1 Antibody (C-term)

Gene Names
SLC29A1; ENT1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ENT1; Polyclonal Antibody; ENT1 Antibody (C-term); Equilibrative nucleoside transporter 1; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative NBMPR-sensitive nucleoside transporter; Nucleoside transporter; es-type; Solute carrier family 29 member 1; SLC29A1; anti-SLC29A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the C-terminal region of human ENT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.31 (varies by lot)
Sequence Positions
402-431
Sequence Length
535
Applicable Applications for anti-SLC29A1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC~~1:25
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. brain section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. brain section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. colon section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. colon section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded R. brain section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded R. brain section using ENT1(Slc29a1) (C-term). MBS9214223 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysate from human heart tissue lysate, using ENT1(Slc29a1) Antibody (C-term). MBS9214223 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB) (Western blot analysis of lysate from human heart tissue lysate, using ENT1(Slc29a1) Antibody (C-term). MBS9214223 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB)

(Western blot analysis of ENT1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). ENT1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ENT1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). ENT1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-SLC29A1 antibody
ENT1 is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.
Product Categories/Family for anti-SLC29A1 antibody
References
Bone,D.B.,Am. J. Physiol. Heart Circ. Physiol. 293 (6), H3325-H3332 (2007) Damaraju,V.L., Am. J. Physiol. Renal Physiol. 293 (1), F200-F211 (2007) Abdulla,P.,Nucleosides Nucleotides Nucleic Acids 26 (1), 99-110 (2007) Sundaram,M., J. Biol. Chem. 276 (48), 45270-45275 (2001)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50219
NCBI Official Synonym Full Names
solute carrier family 29 (equilibrative nucleoside transporter), member 1
NCBI Official Symbol
SLC29A1
NCBI Official Synonym Symbols
ENT1
NCBI Protein Information
equilibrative nucleoside transporter 1
UniProt Protein Name
Equilibrative nucleoside transporter 1
UniProt Gene Name
SLC29A1
UniProt Synonym Gene Names
ENT1; Equilibrative NBMPR-sensitive nucleoside transporter
UniProt Entry Name
S29A1_HUMAN

NCBI Description

This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Protein type: Membrane protein, integral; Transporter, SLC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: nucleoside transmembrane transporter activity

Biological Process: lactation; nucleoside transport; uridine transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; sleep; regulation of excitatory postsynaptic membrane potential; transmembrane transport

Research Articles on SLC29A1

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Product Notes

The SLC29A1 slc29a1 (Catalog #AAA9214223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 402-431. The ENT1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ENT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the SLC29A1 slc29a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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