ECGF (Crude Extract, cell culture grade) + Heparin Crude Extract | ECGF crude extract

Bovine ECGF (Crude Extract, cell culture grade) + Heparin

Cat. Num. AAA691459

• Reactivity: Bovine
• Applications: Cell Culture
• Purity: Crude extract
• Synonyms: ECGF (Crude Extract; cell culture grade) + Heparin; Bovine ECGF (Crude Extract; Bovine Endothelial Cell Growth Factor (ECGF) (Cell culture grade + Heparin); ECGF crude extract

• Host: Cattle
• Reactivity: Bovine
• Specificity: Bovine ECGF is effective on mouse, bovine and human cells.
• Purity/Purification: Crude extract
• Form/Format: Freeze dried

• Applicable Applications for ECGF crude extract: Cell Culture
• Biological Activity: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 ug/ml, optimal concentration with heparin (30 ug/ml) is about 12 ug/ml.
• Buffer: H2O, w/o preservative
• Preparation and Storage: Also stable at 4 degree C for several weeks it is recommended to store the product below 0 degree C. After reconstitution the product shout be stored in aliquots at -20 degree C to -70 degree C.

Testing Data

Related Product Information for ECGF crude extract

Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate. Endothelial cells from human umbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows to cultivate endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.

References

1. Maciag T (1982) JBC 257:5333 2. Olander J (1980) In Vitro 6:209 3. Folkman J (1980) Nature 288:551 4. Evans CH (1982) JNCI 68:127 5. Pintus C (1983) J Immuno Meth 61:195 6. Maciag T (1979) PNAS 6:5674 7. Thornton SC (1983) Science 222:623 8. Ransom JH (1986) Methods Enzymol 121:293 9. Schniedermann et al. BMC Cell Biology 2010