Western Blot (WB)
(ENOA Antibody (N-term) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow).)
Rabbit anti-Human ENOA Polyclonal Antibody | anti-eno antibody
ENOA, NT (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Western Blot (WB)
(ENOA Antibody (N-term) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow).)
Western Blot (WB)
(ENOA Antibody (N-term) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow).)
Flow Cytometry (FC/FACS)
(ENOA Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ENOA Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ENOA Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENOA primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis
By similarity. HAMAP MF_00318
Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O. HAMAP MF_00318
Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer
Enzyme regulation: The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein
By similarity. HAMAP MF_00318
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. HAMAP MF_00318
Subcellular location: Cytoplasm. Secreted. Cell surface. Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial cell surface
By similarity. HAMAP MF_00318
Sequence similarities: Belongs to the enolase family.
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Product Notes
The eno eno (Catalog #AAA627408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENOA, NT (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENOA can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the eno eno for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENOA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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