Rabbit anti-Human ENO1 Polyclonal Antibody | anti-ENO1 antibody

ENO1, ID (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1)

Cat. Num. AAA6002063

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: ENO1; Polyclonal Antibody; ID (Alpha-enolase; 2-phospho-D-glycerate Hydro-lyase; Non-neural Enolase; NNE; Enolase 1; Phosphopyruvate Hydratase; C-myc Promoter-binding Protein; MBP-1; MPB-1; Plasminogen-binding Protein; MPB1; MBPB1; ENO1L1); Anti -ENO1; anti-ENO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Alpha Enolase.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ENO1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunohistochemistry.; Dilution: ELISA: 1:50-1:100; Western Blot: 1:50-1:100; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to the internal region of human Alpha Enolase (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Adrenal (formalin-fixed, paraffin-embedded) stained with ENO1 antibody followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Breast (formalin-fixed, paraffin-embedded) stained with ENO1 antibody followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-ENO1 antibody

ENO1 is one of three enolase isoenzymes found in mammals; it is alpha-enolase, a homodimeric soluble enzyme, and also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Product Categories/Family for anti-ENO1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 39644850
• NCBI GeneID: 2023
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• Molecular Weight: 47,169 Da
• NCBI Official Full Name: ENO1 protein, partial
• NCBI Official Synonym Full Names: enolase 1, (alpha)
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Alpha-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN

NCBI Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Lyase; EC 4.2.1.11; Transcription, coactivator/corepressor; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane; M band; nucleus; cytosol

Molecular Function: protein binding; DNA binding; magnesium ion binding; phosphopyruvate hydratase activity; transcription corepressor activity; transcription factor activity

Biological Process: glycolysis; transcription, DNA-dependent; response to virus; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; gluconeogenesis