Immunofluorescence (IF)
(MBS9605496 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human ENDOGL1 Polyclonal Antibody | anti-ENDOGL1 antibody
ENDOGL1 Antibody
Gene Names
EXOG; ENGL; ENGLA; ENGLB; ENGL-a; ENGL-b; ENDOGL1; ENDOGL2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ENDOGL1; Polyclonal Antibody; ENDOGL1 Antibody; Endo G like 1; Endo G-like 1; Endo/exonuclease (5'-3'); endonuclease G like; ENDOGL 1; ENDOGL2; Endonuclease G like 1; Endonuclease G like 2; Endonuclease G-like 1; ENGL a; ENGL; ENGL B; ENGLA; ENGLB; Exog; EXOG_HUMAN; MGC125944; MGC125945; mitochondrial; Nuclease EXOG; Nuclease EXOG mitochondrial; anti-ENDOGL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ENDOGL1 antibody detects endogenous levels of total ENDOGL1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
318
Applicable Applications for anti-ENDOGL1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Dog, Chicken
Similarity
Pig (80%), Bovine (80%), Sheep (80%), Dog (80%), Chicken (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605496 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605496 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells using ENDOGL1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells using ENDOGL1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using ENDOGL1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using ENDOGL1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Related Product Information for anti-ENDOGL1 antibody
Function: Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.
Subunit Structure: Homodimer.
Similarity: Belongs to the DNA/RNA non-specific endonuclease family.
Subunit Structure: Homodimer.
Similarity: Belongs to the DNA/RNA non-specific endonuclease family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 41 kD
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
nuclease EXOG, mitochondrial isoform 2
NCBI Official Synonym Full Names
exo/endonuclease G
NCBI Official Symbol
EXOG
NCBI Official Synonym Symbols
ENGL; ENGLA; ENGLB; ENGL-a; ENGL-b; ENDOGL1; ENDOGL2
NCBI Protein Information
nuclease EXOG, mitochondrial
UniProt Protein Name
Nuclease EXOG, mitochondrial
UniProt Gene Name
EXOG
UniProt Synonym Gene Names
ENDOGL1; ENDOGL2; ENGL; Endo G-like 1
NCBI Description
This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18. [provided by RefSeq, Feb 2009]
Uniprot Description
Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.
Research Articles on ENDOGL1
Similar Products
Product Notes
The ENDOGL1 exog (Catalog #AAA9605496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENDOGL1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENDOGL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ENDOGL1 exog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENDOGL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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