Chicken anti-Human ENC-2 Polyclonal Antibody | anti-KLHL25 antibody

ENC-2 Antibody

Cat. Num. AAA150793

• Gene Names: KLHL25; ENC2; ENC-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ENC-2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ENC-2; Polyclonal Antibody; ENC-2 Antibody; ENC2; Kelch-like protein 25; Ectoderm-neural cortex protein 2; kelch-like 25 (Drosophila); anti-KLHL25 antibody

• Host: Chicken
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgY
• Purity/Purification: ENC-2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 589

• Applicable Applications for anti-KLHL25 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ENC-2 antibody was raised against a 14 amino acid synthetic peptide near the center of human ENC-2.
• Buffer: ENC-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ENC-2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunocytochemistry (IHC)

(Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 μg/mL.)

Related Product Information for anti-KLHL25 antibody

ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation. The exact function of ENC-2 has yet to be determined, but it has been suggested to be involved in the differentiation of neuronal precursor cells into neurons and astrocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 55418570
• NCBI GeneID: 64410
• UniProt Accession #: Q9H0H3; B2RDH2; B3KRT7
• Molecular Weight: 65,923 Da
• NCBI Official Full Name: ENC2
• NCBI Official Synonym Full Names: kelch-like family member 25
• NCBI Official Symbol: KLHL25
• NCBI Official Synonym Symbols: ENC2; ENC-2
• NCBI Protein Information: kelch-like protein 25; kelch-like 25; ectodermal-neural cortex 2; BTB/POZ KELCH domain protein; ectoderm-neural cortex protein 2
• UniProt Protein Name: Kelch-like protein 25
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL25
• UniProt Synonym Gene Names: ENC2; ENC-2
• UniProt Entry Name: KLH25_HUMAN

Uniprot Description

KLHL25: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated)

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytoplasm

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; regulation of translational initiation