Western Blot (WB)
(Figure 1. Western blot analysis using KLHL25 mAb against KLHL25-hIgGFc transfected HEK293 cell.)
Mouse anti-Human KLHL25 Antibody | anti-KLHL25 antibody
KLHL25 Antibody
Gene Names
KLHL25; ENC2; ENC-2
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
KLHL25; KLHL25 Antibody; ENC2; ENC-2; FLJ12587; FLJ45015; anti-KLHL25 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2b
Clone Number
1B7
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
589
Applicable Applications for anti-KLHL25 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:10000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human KLHL25 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using KLHL25 mAb against KLHL25-hIgGFc transfected HEK293 cell.)
Western Blot (WB)
(Figure 1. Western blot analysis using KLHL25 mAb against KLHL25-hIgGFc transfected HEK293 cell.)
Related Product Information for anti-KLHL25 antibody
KLHL25 (ectoderm-neural cortex protein 2, ENC2) is a cytoplasmic protein that contains six Kelch regions and a single BTB (POZ) domain. KLHL25 is highly homologus to another Kelch-like protein, ENC1, and it is believed to operate in a manner similar to other Kelch-domain containing proteins. Kelch-domain repeat containing proteins often act as modifiers of Actin fibers. Expressed early in embryogenesis, ENC1 helps to mediate neuronal process formation. It also appears to have a role in neural crest cell differentiation. KLHL25 likely functions as a substrate specific adapter for protein ubiquitinating complexes. KLHL25 is expressed in most tissues with highest expression in brain and liver.
Product Categories/Family for anti-KLHL25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65kDa
NCBI Official Full Name
kelch-like protein 25
NCBI Official Synonym Full Names
kelch like family member 25
NCBI Official Symbol
KLHL25
NCBI Official Synonym Symbols
ENC2; ENC-2
NCBI Protein Information
kelch-like protein 25
UniProt Protein Name
Kelch-like protein 25
Protein Family
UniProt Gene Name
KLHL25
UniProt Synonym Gene Names
ENC2; ENC-2
Uniprot Description
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.
Research Articles on KLHL25
Similar Products
Product Notes
The KLHL25 klhl25 (Catalog #AAA850701) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KLHL25 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLHL25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the KLHL25 klhl25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL25, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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