Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (MBS806830). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (MBS806830) at 1:1000.)
Rabbit ENaC Alpha Polyclonal Antibody | anti-Scnn1a antibody
ENaC Alpha Antibody
Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (MBS806830). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (MBS806830) at 1:1000.)
Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (MBS806830). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (MBS806830) at 1:1000.)
Western Blot (WB)
(Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (MBS806830). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (MBS806830) at 1:1000.)
Western Blot (WB)
(Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (MBS806830). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (MBS806830) at 1:1000.)
Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
NCBI and Uniprot Product Information
NCBI Description
acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006]
Uniprot Description
ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, sodium; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel
Cellular Component: cortical actin cytoskeleton; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; cytosol; external side of plasma membrane
Molecular Function: amiloride-sensitive sodium channel activity; WW domain binding; actin binding; sodium channel activity
Biological Process: regulation of body fluid levels; sensory perception of taste; regulation of blood pressure; sodium ion transport; sodium ion homeostasis; multicellular organismal water homeostasis
Research Articles on Scnn1a
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Product Notes
The Scnn1a scnn1a (Catalog #AAA806830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENaC Alpha Antibody reacts with Human, Rat, Mouse, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's ENaC Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB (1:1000), ICC/IF (1:400), IHC (1:25); optimal dilutions for assays should be determined by the user. Researchers should empirically determine the suitability of the Scnn1a scnn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENaC Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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