Rabbit anti-Rat, Mouse ENaC Alpha Polyclonal Antibody | anti-Scnn1a antibody

ENaC Alpha Antibody: ATTO 488

Cat. Num. AAA806430

• Reactivity: Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: ENaC Alpha; Polyclonal Antibody; ENaC Alpha Antibody: ATTO 488; Scnn1a; anti-Scnn1a antibody

• Host: Rabbit
• Reactivity: Rat, Mouse
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 699

• Applicable Applications for anti-Scnn1a antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: 1:1000 (WB), 1:400 (IF)
• Conjugate: ATTO 488
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide.
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)

Western Blot (WB)

(Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-403). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-403) at 1:1000.)

Related Product Information for anti-Scnn1a antibody

Background Info: Detects ~85kDa

Scientific Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

Product Categories/Family for anti-Scnn1a antibody

Ion Channels; Transporters

References

1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.

NCBI and Uniprot Product Information

• NCBI GI #: 13928742
• NCBI GeneID: 25122
• NCBI Accession #: NP_113736
• NCBI GenBank Nucleotide #: NM_031548.2
• UniProt Accession #: Q6IRJ1; P37089
• Molecular Weight: 79,019 Da
• NCBI Official Full Name: amiloride-sensitive sodium channel subunit alpha
• NCBI Official Synonym Full Names: sodium channel, non-voltage-gated 1 alpha subunit
• NCBI Official Symbol: Scnn1a
• NCBI Protein Information: amiloride-sensitive sodium channel subunit alpha; SCNEA; alpha-ENaC; alpha-NaCH; epithelial Na(+) channel subunit alpha; epithelial sodium channel alpha subunit; sodium channel, nonvoltage-gated 1 alpha; nonvoltage-gated sodium channel 1 subunit alpha; sodium channel, nonvoltage-gated, type I, alpha
• UniProt Protein Name: Amiloride sensitive sodium channel alpha1 subunit
• Protein Family: Amiloride-sensitive sodium channel
• UniProt Gene Name: Scnn1a
• UniProt Synonym Gene Names: rCG_30182
• UniProt Entry Name: Q6IRJ1_RAT

NCBI Description

acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006]

Uniprot Description

ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, sodium; Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass

Cellular Component: cortical actin cytoskeleton; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; cytosol; external side of plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; WW domain binding; actin binding; sodium channel activity

Biological Process: regulation of body fluid levels; sensory perception of taste; regulation of blood pressure; sodium ion transport; sodium ion homeostasis; multicellular organismal water homeostasis