Western Blot (WB)
(Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.)
Mouse anti-Mouse; Rat ENaC alpha Monoclonal Antibody | anti-ENaC alpha antibody
ENaC alpha Antibody, Clone 14E10
Gene Names
Scnn1a; ENaCA
Reactivity
Mouse; Rat
Applications
Western Blot, Immunohistochemistry
Purity
Protein G Purified
Synonyms
ENaC alpha; Monoclonal Antibody; ENaC alpha Antibody; Clone 14E10; Mouse Anti-Rat ENaC alpha Monoclonal IgG1; SCNN1A Antibody; Epithelial Sodium Channel-alpha Antibody; Epithelial Sodium Channel alpha Antibody; Alpha ENaC 2 Antibody; Alpha ENaC Antibody; Alpha NaCH Antibody; Alpha-ENaC Antibody; Amiloride sensitive epithelial sodium channel alpha subunit Antibody; Amiloride sensitive sodium channel subunit alpha Antibody; Amiloride-sensitive sodium channel subunit alpha Antibody; ENaCa Antibody; ENaCalpha Antibody; Epithelial Na(+) channel subunit alpha Antibody; Epithelial Na+ channel subunit alpha Antibody; FLJ21883 Antibody; Nonvoltage gated sodium channel 1 subunit alpha Antibody; Nonvoltage-gated sodium channel 1 subunit alpha Antibody; SCNEA Antibody; SCNN 1 Antibody; SCNN1 Antibody; SCNNA_HUMAN Antibody; Sodium channel nonvoltage gated 1 alpha Antibody; anti-ENaC alpha antibody
Host
Mouse
Reactivity
Mouse; Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14E10
Specificity
Detects ~85kDa.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
699
Applicable Applications for anti-ENaC alpha antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IHC: 1:150
IHC: 1:150
Target
ENaC alpha
Immunogen
Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
Immunogen Species
Rat
Cellular Localization
Apical Cell Membrane
Conjugate
Unconjugated
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.)
Western Blot (WB)
(Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 14E10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.)
Related Product Information for anti-ENaC alpha antibody
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Product Categories/Family for anti-ENaC alpha antibody
References
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,019 Da
NCBI Official Full Name
amiloride-sensitive sodium channel subunit alpha
NCBI Official Synonym Full Names
sodium channel epithelial 1 alpha subunit
NCBI Official Symbol
Scnn1a
NCBI Official Synonym Symbols
ENaCA
NCBI Protein Information
amiloride-sensitive sodium channel subunit alpha
UniProt Protein Name
Amiloride sensitive sodium channel alpha1 subunit
UniProt Gene Name
Scnn1a
NCBI Description
acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006]
Research Articles on ENaC alpha
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Product Notes
The ENaC alpha scnn1a (Catalog #AAA808322) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ENaC alpha Antibody, Clone 14E10 reacts with Mouse; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ENaC alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:150. Researchers should empirically determine the suitability of the ENaC alpha scnn1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENaC alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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