Rabbit anti-Human EMD / Emerin Polyclonal Antibody | anti-EMD antibody

Rabbit Polyclonal to Human EMD / Emerin

Cat. Num. AAA248338

• Gene Names: EMD; STA; EDMD; LEMD5
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Ammonium sulfate precipitation
• Synonyms: EMD / Emerin; Polyclonal Antibody; Rabbit Polyclonal to Human EMD / Emerin; Anti-EMD / Emerin Antibody (N-Terminus) IHC-plus; EMD; EDMD; Emerin; LEM domain containing 5; LEMD5; STA; Human EMD; anti-EMD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 254

• Applicable Applications for anti-EMD antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide selected from the N-terminal region of human EMD.
• Immunogen Type: Synthetic peptide
• Immunogen: EMD / Emerin antibody was raised against kLH conjugated synthetic peptide selected from the N-terminal region of human EMD.
• Antigen Modification: N-Terminus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Flow Cytometry (FC/FACS)

(Flow cytometry of 293 cells using EMD Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of EMD antibody (N-term) in 293 cell line lysates (35 ug/lane). EMD (arrow) was detected using the purified Pab.)

NCBI and Uniprot Product Information

• NCBI GI #: 4557553
• NCBI GeneID: 2010
• NCBI Accession #: NP_000108.1
• NCBI GenBank Nucleotide #: NM_000117.2
• UniProt Accession #: P50402; Q6FI02
• Molecular Weight: 28,994 Da
• NCBI Official Full Name: emerin
• NCBI Official Synonym Full Names: emerin
• NCBI Official Symbol: EMD
• NCBI Official Synonym Symbols: STA; EDMD; LEMD5
• NCBI Protein Information: emerin; LEM domain containing 5
• UniProt Protein Name: Emerin
• Protein Family: Emerin
• UniProt Gene Name: EMD
• UniProt Synonym Gene Names: EDMD; STA
• UniProt Entry Name: EMD_HUMAN

NCBI Description

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]

Uniprot Description

emerin: a single pass membrane protein that spans the inner nuclear membrane. A serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It interacts with lamins A and C, and mediates membrane anchorage to the cytoskeleton. Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through an exportin 1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Colocalized with BAF at the central region of the assembling nuclear rim, near spindle-attachment sites. Hyper-phosphorylated on tyrosine in cells overexpressing HER2. Directly phosphorylated by Src and Abl. Phosphorylated by Src at Y59, Y74 and Y95. May function as a downstream effector and signal integrator for tyrosine kinase signaling pathways at the nuclear envelope. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Protein type: Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nuclear outer membrane; microtubule; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear inner membrane; nuclear envelope

Molecular Function: protein binding; beta-tubulin binding; actin binding

Biological Process: positive regulation of protein export from nucleus; muscle development; mitotic nuclear envelope reassembly; muscle contraction; negative regulation of fibroblast proliferation; mitotic nuclear envelope disassembly; mitotic cell cycle

Disease: Emery-dreifuss Muscular Dystrophy 1, X-linked