Rabbit anti-Human EMAP-II/AIMP1 Polyclonal Antibody | anti-AIMP1 antibody

Anti-EMAP-II/AIMP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103394

• Gene Names: AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: EMAP-II/AIMP1; Polyclonal Antibody; Anti-EMAP-II/AIMP1 Antibody; Rabbit Polyclonal; EMAP-II/AIMP1 Antibody; Rabbit PAb; Antigen Affinity Purified; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; Anti-EMAP2 Antibody; Anti-EMAPII Antibody; Anti-HLD3 Antibody; Anti-p43 Antibody; Anti-SCYE1 Antibody; anti-AIMP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human EMAP-II/AIMP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-AIMP1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:10,000; ICC/IF: 1:200-1:1000; IP: 1-5uL/mg of lysate
• Immunogen: Recombinant Human EMAP-II/AIMP1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human EMAP-II/AIMP1 (rh EMAP-II/AIMP1). EMAP-II/AIMP1 specific IgG was purified by Human EMAP-II/AIMP1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SCYE1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human SCYE1 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human SCYE1 in human pancreas with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of SCYE1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SCYE1 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(SCYE1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-SCYE1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SCYE1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :35 kDa)

Related Product Information for anti-AIMP1 antibody

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, also known as Multisynthase complex auxiliary component p43, Endothelial monocyte-activating polypeptide II, AIMP1, EMAP2 and SCYE1, is a nucleus protein which contains one tRNA-binding domain. AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. AIMP1 negatively regulates TGF-beta signaling via stabilization of Smurf2. It suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-beta signaling. Recently, it been demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response. AIMP1 is known as a cytokine working in the control of angiogenesis, inflammation, and wound healing. AIMP1 is secreted from the pancreas upon glucose starvation, and it also plays a glucagon-like role in glucose homeostasis. Although AIMP1 was identified as a component of the macromolecular aminoacyl tRNA synthetase complex involved in the cellular translation process, it was also found to be secreted as a cytokine having complex physiological functions. Among these, AIMP1's angiostatic and immune stimulating activities suggest its potential use as a novel antitumor therapeutic protein. AIMP1 may exert its antitumor activity by inducing tumor-suppressing cytokines. Thus, AIMP1 may be useful as a novel anti-tumor agent.

References

Lee YS, et al. (2006) Antitumor activity of the novel human cytokine AIMP1 in an in vivo tumor model. Mol Cells. 21(2): 213-7.Park SG, et al. (2006) Hormonal activity of AIMP1/p43 for glucose homeostasis. Proc Natl Acad Sci U S A. 103(40): 14913-8.Kim E, et al. (2008) AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability. J Immunol. 180(5): 2894-902.Lee YS, et al. (2008) AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2. Biochem Biophys Res Commun. 371(3): 395-400.Han JM, et al. (2010) Antitumor activity and pharmacokinetic properties of ARS-interacting multi-functional protein 1 (AIMP1/p43). Cancer Lett. 287(2): 157-64.

NCBI and Uniprot Product Information

• NCBI GI #: 215490007
• NCBI GeneID: 9255
• NCBI Accession #: NP_004748.2
• NCBI GenBank Nucleotide #: NM_004757
• Molecular Weight: 37,039 Da
• NCBI Official Full Name: Homo sapiens aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• NCBI Official Symbol: AIMP1
• NCBI Official Synonym Symbols: p43; HLD3; EMAP2; SCYE1; EMAPII
• NCBI Protein Information: aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; multisynthase complex auxiliary component p43
• Protein Family: Aminoacyl tRNA synthase complex-interacting multifunctional protein

NCBI Description

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]