Mouse anti-Human ATG4D Monoclonal Antibody | anti-ATG4D antibody

ATG4D (ATG4D, APG4D, AUTL4, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D) (AP)

Cat. Num. AAA6120792

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: ATG4D; Monoclonal Antibody; ATG4D (ATG4D; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D) (AP); Autophagy-related protein 4 homolog D; anti-ATG4D antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 222CT15.4.1
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ATG4D antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000; WB: 1:100-1000; Applications are based on unconjugated antibody.
• Immunogen: ATG4D Mab is generated from mouse immunized with ATG4D recombinant protein.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Western Blot (WB)

(ATG4D/MB10250 antibody western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ATG4D/MB10250 antibody detected the ATG4D/MB10250 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ATG4D Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-ATG4D antibody

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

Product Categories/Family for anti-ATG4D antibody

Antibodies; Autophagy

References

Betin, V.M., et al. J. Cell. Sci. 122 (PT 14), 2554-2566 (2009) Lamesch, P., et al. Genomics 89(3):307-315(2007) Marino, G., et al. J. Biol. Chem. 278(6):3671-3678(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 27903825
• NCBI GeneID: 84971
• NCBI Accession #: NP_116274.3
• NCBI GenBank Nucleotide #: NM_032885.4
• UniProt Accession #: Q86TL0
• Molecular Weight: 52,922 Da
• NCBI Official Full Name: cysteine protease ATG4D
• NCBI Official Synonym Full Names: autophagy related 4D, cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D; autophagin-4; APG4 autophagy 4 homolog D; AUT-like 4 cysteine endopeptidase; ATG4 autophagy related 4 homolog D; AUT-like 4, cysteine endopeptidase; autophagy-related protein 4 homolog D; cysteine protease involved in autophagy; a
• UniProt Protein Name: Cysteine protease ATG4D
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4
• UniProt Entry Name: ATG4D_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.

Protein type: EC 3.4.22.-; Ubiquitin conjugating system; Protease; Autophagy

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: mitochondrial matrix; cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity

Biological Process: protein delipidation; apoptosis; mitochondrion degradation; protein processing; C-terminal protein lipidation; protein targeting to membrane; proteolysis; cellular response to nitrogen starvation; autophagic vacuole formation