Rabbit EIF4H Polyclonal Antibody | anti-EIF4H antibody

Anti-EIF4H Antibody, Rabbit Polyclonal

Cat. Num. AAA8103654

• Gene Names: EIF4H; WSCR1; WBSCR1; eIF-4H
• Reactivity: Human; Predicted to React with: Mouse, Rat, Pongo abelii (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: EIF4H; Polyclonal Antibody; Anti-EIF4H Antibody; Rabbit Polyclonal; EIF4H Antibody; Rabbit PAb; Antigen Affinity Purified; eukaryotic translation initiation factor 4H; Anti-eIF-4H Antibody; Anti-WBSCR1 Antibody; Anti-WSCR1 Antibody; anti-EIF4H antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Pongo abelii (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human EIF4H
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EIF4H antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the center region of the Human EIF4H
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human EIF4H, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-EIF4H rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:27 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of EIF4H in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human EIF4H polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

NCBI and Uniprot Product Information

• NCBI GI #: 11559923
• NCBI GeneID: 7458
• NCBI Accession #: NP_071496.1
• NCBI GenBank Nucleotide #: NM_022170.1
• UniProt Accession #: Q15056; A8K3R1; D3DXF6; D3DXF8
• Molecular Weight: 25,200 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4H isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4H
• NCBI Official Symbol: EIF4H
• NCBI Official Synonym Symbols: WSCR1; WBSCR1; eIF-4H
• NCBI Protein Information: eukaryotic translation initiation factor 4H; Williams-Beuren syndrome chromosome region 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4H
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4H
• UniProt Synonym Gene Names: KIAA0038; WBSCR1; WSCR1; eIF-4H
• UniProt Entry Name: IF4H_HUMAN

NCBI Description

This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

EIF4H: eukaryotic translation initiation factor 4H stimulates protein translation. May stimulate the activities of EIF4A, EIF4B and EIF4F during the steps involving mRNA binding and utilization in the initiation of protein biosynthesis. Binds mRNA. Two alternatively spliced isoforms have been described. The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.

Protein type: Translation; RNA-binding; Translation initiation

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: membrane; perinuclear region of cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; RNA binding; translation initiation factor activity; nucleotide binding

Biological Process: developmental growth; cellular protein metabolic process; translation; viral reproduction; sexual reproduction; translational initiation; gene expression; regulation of translational initiation

Disease: Williams-beuren Syndrome