Western Blot (WB)
(Western blot analysis of HDAC2 (arrow) using rabbit polyclonal HDAC2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC2 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human HDAC2 Polyclonal Antibody | anti-HDAC2 antibody
HDAC2 Antibody (C-term)
IF:~~1:10~50
IHC-P:~~1:10~50
Western Blot (WB)
(Western blot analysis of HDAC2 (arrow) using rabbit polyclonal HDAC2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC2 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of HDAC2 (arrow) using rabbit polyclonal HDAC2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC2 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of anti-HDAC2 Antibody (C-term) in 293 cell line lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-HDAC2 Antibody (C-term) in 293 cell line lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-HDAC2 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-HDAC2 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HDAC2 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HDAC2 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Immunohistochemistry (IHC)-Paraffin
(HDAC2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(HDAC2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)
Background: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Uniprot Description
HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Protein type: Deacetylase; EC 3.5.1.98; Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: cytoplasm; ESC/E(Z) complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex
Molecular Function: chromatin binding; deacetylase activity; enzyme binding; heat shock protein binding; histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; sequence-specific DNA binding; transcription factor binding
Biological Process: ATP-dependent chromatin remodeling; behavioral response to ethanol; blood coagulation; chromatin remodeling; circadian regulation of gene expression; dendrite development; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; maintenance of chromatin silencing; negative regulation of apoptosis; negative regulation of DNA binding; negative regulation of MHC class II biosynthetic process; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of interleukin-1 production; positive regulation of oligodendrocyte differentiation; positive regulation of proteolysis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; response to amphetamine; response to caffeine; response to cocaine; response to drug; response to hyperoxia; response to lipopolysaccharide; response to nicotine; transcription, DNA-dependent
Research Articles on HDAC2
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Product Notes
The HDAC2 hdac2 (Catalog #AAA9217080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB:~~1:1000 IF:~~1:10~50 IHC-P:~~1:10~50. Researchers should empirically determine the suitability of the HDAC2 hdac2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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