Rabbit anti-Human, Mouse AMPH Polyclonal Antibody | anti-AMPH antibody

AMPH polyclonal antibody

Cat. Num. AAA3005672

• Gene Names: AMPH; AMPH1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AMPH; Polyclonal Antibody; AMPH polyclonal antibody; Amphiphysin; AMPH1; anti-AMPH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AMPH polyclonal antibody detects endogenous levels of AMPH protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Sequence Length: 695

• Applicable Applications for anti-AMPH antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to Human AMPH.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AMPH polyclonal antibody at 1:500 dilutionLane1:U-87MG whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:SHSY5Y whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug))

Related Product Information for anti-AMPH antibody

Amphiphysin is a brain-enriched protein that exhibits N-terminal lipid interaction and functions as a dimer. Amphiphysin contains a membrane bending BAR domain, a middle Clathrin and adaptor binding domain and a C-terminal SH3 domain. In the brain, Amphiphysin I and II form heterodimers that bind to the Clathrin-associated GTPase Dynamin via their SH3 domains. This association is essential for synaptic vesicle recycling in neurons, as it precedes the binding of Dynamin to the Clathrin-coated pits and the subsequent vesicle budding. In other tissues, Amphiphysin may play a key role in other membrane bending and curvature stabilization events. The mammalian Amphiphysins, Amphiphysin I and Amphiphysin II, have similar overall structure. An ubiquitous splice form of Amphiphysin II that does not contain Clathrin or adaptor interactions is highly expressed in muscle tissue and is involved in the formation and stabilization of the T tubule network.

NCBI and Uniprot Product Information

• NCBI GI #: 4502081
• NCBI GeneID: 273
• NCBI Accession #: NP_001626.1
• NCBI GenBank Nucleotide #: NP_001626.1
• UniProt Accession #: P49418
• Molecular Weight: ~ 105kDa
• NCBI Official Full Name: amphiphysin isoform 1
• NCBI Official Synonym Full Names: amphiphysin
• NCBI Official Symbol: AMPH
• NCBI Official Synonym Symbols: AMPH1
• NCBI Protein Information: amphiphysin
• UniProt Protein Name: Amphiphysin
• Protein Family: Amphinase
• UniProt Gene Name: AMPH
• UniProt Synonym Gene Names: AMPH1
• UniProt Entry Name: AMPH_HUMAN

NCBI Description

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]

Uniprot Description

amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: synaptic vesicle; synaptic vesicle membrane; cell junction; actin cytoskeleton

Molecular Function: protein binding; phospholipid binding

Biological Process: synaptic transmission; synaptic vesicle endocytosis; endocytosis; learning