Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: K562 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 minutes)
Rabbit anti-Human EIF3I Polyclonal Antibody | anti-EIF3I antibody
EIF3I Antibody
Gene Names
EIF3I; TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
EIF3I; Polyclonal Antibody; EIF3I Antibody; TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta; anti-EIF3I antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total EIF3I protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
325
Applicable Applications for anti-EIF3I antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen
Fusion protein of human EIF3I
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: K562 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 minutes)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: K562 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 minutes)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20. (Original magnification: ¡200))
Related Product Information for anti-EIF3I antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
Product Categories/Family for anti-EIF3I antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,502 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit I
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit I
NCBI Official Symbol
EIF3I
NCBI Official Synonym Symbols
TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit I
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit I
UniProt Gene Name
EIF3I
UniProt Synonym Gene Names
eIF3i; TRIP-1
UniProt Entry Name
EIF3I_HUMAN
Uniprot Description
eIF3-beta: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor.
Protein type: Translation; Translation initiation
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex
Molecular Function: protein binding; translation initiation factor activity
Biological Process: translational initiation
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Product Notes
The EIF3I eif3i (Catalog #AAA9416023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3I Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the EIF3I eif3i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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