EIF3I recombinant protein

Recombinant EIF3I

Cat. Num. AAA201140

• Gene Names: EIF3I; TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: EIF3I; Recombinant EIF3I; eIF3-beta; eIF3-p36; EIF3S2; PRO2242; TRIP-1; TRIP1; Eukaryotic translation initiation factor 3 subunit I; eIF3i; TGF-beta receptor-interacting protein 1; eIF-3-beta; EIF3I recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 20mM Tris-HCl Buffer (pH 8.0) Containing 0.1M NaCl, 1mM DTT, 40% Glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 1-325aa
• Sequence: MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG FDFGGNIIMF STDKQMGYQC FVSFFDLRDP SQIDNNEPYM KIPCNDSKIT SAVWGPLGEC IIAGHESGEL NQYSAKSGEV LVNVKEHSRQ INDIQLSRDM TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPN YDHVVLGGGQ EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY SSGGEDGYVR IHYFDPQYFE FEFEA HHHHH H
• Sequence Length: 325

• Applicable Applications for EIF3I recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Preparation and Storage: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for EIF3I recombinant protein

EIF3I, also known as eukaryotic translation initiation factor 3 subunit I, is a multiprotein complex that functions during the initiation phase of eukaryotic translation. This protein is one out of 13 subunits of the mammalian EIF3 complex which associated with the 40S ribosome and facilitates the formation of 43S pre-initiation complex. This complex stimulates nearly all steps of translation initiation. These factors also appear to participate in other phases of translation, such as recycling, where it promotes the splitting of post-termination ribosomes. Also, it is shown to act as a signaling transducer by bind to and activating AKT1 through preventing PP2A-induced AKT1 dephosphorylation in tumor cells. Recombinant Human EIF3I, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for EIF3I recombinant protein

Epigenomics (Transcription & Translation)

References

Pan W., et al, (2017) Vet. Microbiol. 212:59-66.

NCBI and Uniprot Product Information

• NCBI GI #: 4503513
• NCBI GeneID: 8668
• NCBI Accession #: NP_003748.1
• NCBI GenBank Nucleotide #: NP_003748.1
• UniProt Accession #: Q13347
• Molecular Weight: 37.3kDa (331aa)
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit I
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3 subunit I
• NCBI Official Symbol: EIF3I
• NCBI Official Synonym Symbols: TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit I
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit I
• UniProt Gene Name: EIF3I
• UniProt Entry Name: EIF3I_HUMAN

Uniprot Description

eIF3-beta: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: eukaryotic translation initiation factor 3 complex; cytosol

Molecular Function: protein binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation