Rabbit EIF3H Polyclonal Antibody | anti-EIF3H antibody

EIF3H Rabbit Polyclonal

Cat. Num. AAA768941

• Gene Names: EIF3H; EIF3S3; eIF3-p40; eIF3-gamma
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: EIF3H; Polyclonal Antibody; EIF3H Rabbit Polyclonal; anti-EIF3H antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 352

• Applicable Applications for anti-EIF3H antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Eukaryotic translation initiation factor 3, subunit H
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using MBS768941 (EIF3H antibody) at dilution of 1:100)

Western Blot (WB)

(mouse testis tissue were subjected to SDS PAGE followed by western blot with MBS768941 (EIF3H antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 4503515
• NCBI GeneID: 8667
• NCBI Accession #: NP_003747.1
• NCBI GenBank Nucleotide #: NM_003756.2
• Molecular Weight: 39,930 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit H
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3 subunit H
• NCBI Official Symbol: EIF3H
• NCBI Official Synonym Symbols: EIF3S3; eIF3-p40; eIF3-gamma
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit H
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit H
• UniProt Gene Name: EIF3H
• UniProt Synonym Gene Names: eIF3h
• UniProt Entry Name: EIF3H_HUMAN

Uniprot Description

eIF3S3: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination- dependent manner. Belongs to the eIF-3 subunit H family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: cytosol; eukaryotic translation initiation factor 3 complex; membrane

Molecular Function: protein binding; translation initiation factor activity

Biological Process: formation of translation preinitiation complex; regulation of translational initiation; translational initiation