Rabbit MPRIP Polyclonal Antibody | anti-MPRIP antibody

MPRIP polyclonal antibody

Cat. Num. AAA3000728

• Gene Names: MPRIP; MRIP; RIP3; M-RIP; RHOIP3; p116Rip
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: MPRIP; Polyclonal Antibody; MPRIP polyclonal antibody; KIAA0864; MRIP; RHOIP3; Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip; anti-MPRIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: M-RIP Polyclonal Antibody detects endogenous levels of M-RIP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-MPRIP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; ELISA: 1/5000
• Immunogen: Synthesized peptide derived from the Internal region of human M-RIP.
• Function: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
• Similarity: Contains 1 PH domain.; Contains 2 PH domains.
• Subcellular Location: Colocalizes with F-actin.
• Subunit: Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using M-RIP Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of 293 cells using M-RIP Polyclonal Antibody)

NCBI and Uniprot Product Information

• NCBI GI #: 50980307
• NCBI GeneID: 23164
• NCBI Accession #: NP_055949.2
• NCBI GenBank Nucleotide #: NM_015134.3
• UniProt Accession #: Q6WCQ1; Q3KQZ5; Q5FB94; Q6DHW2; Q7Z5Y2; Q8N390; Q96G40
• Molecular Weight: 116,533 Da
• NCBI Official Full Name: myosin phosphatase Rho-interacting protein isoform 1
• NCBI Official Synonym Full Names: myosin phosphatase Rho interacting protein
• NCBI Official Symbol: MPRIP
• NCBI Official Synonym Symbols: MRIP; RIP3; M-RIP; RHOIP3; p116Rip
• NCBI Protein Information: myosin phosphatase Rho-interacting protein; Rho interacting protein 3
• UniProt Protein Name: Myosin phosphatase Rho-interacting protein
• Protein Family: Myosin phosphatase Rho-interacting protein
• UniProt Gene Name: MPRIP
• UniProt Synonym Gene Names: KIAA0864; MRIP; RHOIP3; M-RIP; RIP3
• UniProt Entry Name: MPRIP_HUMAN

Uniprot Description

RIP3: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F- actin-binding region leads to disassembly of stress fibers in neuronal cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function: protein binding; actin binding