Rabbit anti-Human EIF3G Polyclonal Antibody | anti-EIF3G antibody

EIF3G, ID (EIF3G, EIF3S4, Eukaryotic translation initiation factor 3 subunit G, Eukaryotic translation initiation factor 3 RNA-binding subunit, Eukaryotic translation initiation factor 3 subunit 4, eIF-3-delta, eIF3 p42, eIF3 p44) (APC)

Cat. Num. AAA6295018

• Gene Names: EIF3G; EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF3G; Polyclonal Antibody; ID (EIF3G; EIF3S4; Eukaryotic translation initiation factor 3 subunit G; Eukaryotic translation initiation factor 3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; eIF-3-delta; eIF3 p42; eIF3 p44) (APC); eIF3 p44; anti-EIF3G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EIF3G antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the Central region of human EIF3G.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EIF3G Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).)

Immunohistochemistry (IHC)

(EIF3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EIF3G Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EIF3G antibody

Antibodies; Translation Initiation Factors

References

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008). Masutani, M., et al. EMBO J. 26(14):3373-3383(2007). Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 49472822
• NCBI GeneID: 8666
• NCBI Accession #: NP_003746.2
• NCBI GenBank Nucleotide #: NM_003755.3
• UniProt Accession #: O75821; O14801; Q969U5
• Molecular Weight: 35,611 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit G
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit G
• NCBI Official Symbol: EIF3G
• NCBI Official Synonym Symbols: EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit G; EIF3G; eIF3 p42; eIF3 p44; eIF-3-delta; eIF-3 RNA-binding subunit; eukaryotic translation initiation factor 3 subunit 4; eukaryotic translation initiation factor 3 subunit p42; eukaryotic translation i
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit G
• UniProt Gene Name: EIF3G
• UniProt Entry Name: EIF3G_HUMAN

Uniprot Description

eIF3-delta: eukaryotic translation initiation factor 3 subunit 4. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. This subunit binds to the 18S rRNA. eIF-3 is composed of at least 12 different subunits. Contains 1 RNA recognition motif (RRM) domain.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: eukaryotic translation initiation factor 3 complex; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; translation initiation factor activity; nucleotide binding

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation