Rabbit anti-Human CYP2A13 Polyclonal Antibody | anti-CYP2A13 antibody

CYP2A13, CT (Cytochrome P450 2A13, CYPIIA13) (MaxLight 490)

Cat. Num. AAA6471441

• Gene Names: CYP2A13; CPAD; CYP2A; CYPIIA13
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP2A13; Polyclonal Antibody; CT (Cytochrome P450 2A13; CYPIIA13) (MaxLight 490); anti-CYP2A13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP2A13.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CYP2A13 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CYP2A13 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP2A13 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). CYP2A13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP2A13 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CYP2A13 antibody

Antibodies; Enzymes; Cytochrome

References

1. Smith, G., et.al., Xenobiotica 28(12), 1129-1165 (1998). 2. Nelson, D.R., et.al., Pharmacogenetics 14(1), 1-18 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 13699809
• NCBI GeneID: 1553
• NCBI Accession #: NP_000757
• NCBI GenBank Nucleotide #: NM_000766
• UniProt Accession #: Q16696
• Molecular Weight: 54kDa
• NCBI Official Full Name: cytochrome P450 2A13
• NCBI Official Synonym Full Names: cytochrome P450 family 2 subfamily A member 13
• NCBI Official Symbol: CYP2A13
• NCBI Official Synonym Symbols: CPAD; CYP2A; CYPIIA13
• NCBI Protein Information: cytochrome P450 2A13
• UniProt Protein Name: Cytochrome P450 2A13
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2A13
• UniProt Entry Name: CP2AD_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. Although its endogenous substrate has not been determined, it is known to metabolize 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a major nitrosamine specific to tobacco. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2A5: Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N- dimethylaniline, 2'-methoxyacetophenone, N- nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4- (methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.14.1; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Secondary Metabolites Metabolism - caffeine

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding

Biological Process: coumarin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway