Immunohistochemistry (IHC)
(Rabbit Anti-EIF3A antibodyFormalin Fixed Paraffin Embedded Tissue: Human KidneyPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)
Rabbit EIF3A Polyclonal Antibody | anti-EIF3A antibody
EIF3A antibody - C-terminal region
Gene Names
EIF3A; EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta
Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Synonyms
EIF3A; Polyclonal Antibody; EIF3A antibody - C-terminal region; anti-EIF3A antibody
Host
Rabbit
Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: PPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVR
Sequence Length
1382
Applicable Applications for anti-EIF3A antibody
Western Blot (WB)
Homology
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunohistochemistry (IHC)
(Rabbit Anti-EIF3A antibodyFormalin Fixed Paraffin Embedded Tissue: Human KidneyPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)
Immunohistochemistry (IHC)
(Rabbit Anti-EIF3A antibodyFormalin Fixed Paraffin Embedded Tissue: Human KidneyPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)
Immunohistochemistry (IHC)
(Rabbit Anti-EIF3A antibodyFormalin Fixed Paraffin Embedded Tissue: Human SpleenPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)
Immunohistochemistry (IHC)
(Rabbit Anti-EIF3A antibodyFormalin Fixed Paraffin Embedded Tissue: Human SpleenPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)
Western Blot (WB)
(WB Suggested Anti-EIF3A AntibodyTitration: 1.0 ug/mlPositive Control: Hela Whole CellThere is BioGPS gene expression data showing that EIF3A is expressed in HeLa)
Western Blot (WB)
(WB Suggested Anti-EIF3A AntibodyTitration: 1.0 ug/mlPositive Control: Hela Whole CellThere is BioGPS gene expression data showing that EIF3A is expressed in HeLa)
Related Product Information for anti-EIF3A antibody
This is a rabbit polyclonal antibody against EIF3A. It was validated on Western Blot
Target Description: EIF3A is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Target Description: EIF3A is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Product Categories/Family for anti-EIF3A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
152kDa
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit A
NCBI Official Symbol
EIF3A
NCBI Official Synonym Symbols
EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit A
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit A
Protein Family
UniProt Gene Name
EIF3A
UniProt Synonym Gene Names
EIF3S10; KIAA0139; eIF3a
UniProt Entry Name
EIF3A_HUMAN
Research Articles on EIF3A
1. miRNA-488 participates in eIF3a mediated cisplatin resistance in non-small-cell lung cance cells.
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Product Notes
The EIF3A eif3a (Catalog #AAA3215309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3A antibody - C-terminal region reacts with Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the EIF3A eif3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: PPALSRDRER DRDREREGEK EKASWRAEKD RESLRRTKNE TDEDGWTTVR. It is sometimes possible for the material contained within the vial of "EIF3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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