Western Blot (WB)
(Western blot analysis in mouse testis tissue lysates (35ug/lane) using MBS6000072. This demonstrates MBS6000072 detected the EIF2B5 protein (arrow).)
Rabbit anti-Human, Mouse EIF2B5 Polyclonal Antibody | anti-eif2b5 antibody
EIF2B5, NT (EIF2B5, EIF2BE, Translation initiation factor eIF-2B subunit epsilon, eIF-2B GDP-GTP exchange factor subunit epsilon)
Gene Names
eif2b5; fd14g07; wu:fd14g07
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
EIF2B5; Polyclonal Antibody; NT (EIF2B5; EIF2BE; Translation initiation factor eIF-2B subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon); Anti -EIF2B5; anti-eif2b5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-eif2b5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
EIF2B5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the N-terminal region of human EIF2B5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis in mouse testis tissue lysates (35ug/lane) using MBS6000072. This demonstrates MBS6000072 detected the EIF2B5 protein (arrow).)
Western Blot (WB)
(Western blot analysis in mouse testis tissue lysates (35ug/lane) using MBS6000072. This demonstrates MBS6000072 detected the EIF2B5 protein (arrow).)
Western Blot (WB)
(Western blot analysis in U251 cell line lysates (35ug/lane) using MBS6000072. This demonstrates MBS6000072 detected the EIF2B5 protein (arrow).)
Western Blot (WB)
(Western blot analysis in U251 cell line lysates (35ug/lane) using MBS6000072. This demonstrates MBS6000072 detected the EIF2B5 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue using MBS6000072 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6000072 for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue using MBS6000072 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6000072 for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-eif2b5 antibody
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
Product Categories/Family for anti-eif2b5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Eif2b5 protein
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2B, subunit 5 epsilon
NCBI Official Symbol
eif2b5
NCBI Official Synonym Symbols
fd14g07; wu:fd14g07
NCBI Protein Information
translation initiation factor eIF-2B subunit epsilon
Protein Family
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Product Notes
The eif2b5 (Catalog #AAA6000072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2B5, NT (EIF2B5, EIF2BE, Translation initiation factor eIF-2B subunit epsilon, eIF-2B GDP-GTP exchange factor subunit epsilon) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2B5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the eif2b5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2B5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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