Rabbit anti-Human, Mouse EIF2B4 Polyclonal Antibody | anti-EIF2B4 antibody

EIF2B4, ID (K161) (EIF2B4, EIF2BD, Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta) (AP)

Cat. Num. AAA6294896

• Gene Names: EIF2B4; EIF2B; EIF-2B; EIF2Bdelta
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF2B4; Polyclonal Antibody; ID (K161) (EIF2B4; EIF2BD; Translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta) (AP); eIF-2B GDP-GTP exchange factor subunit delta; anti-EIF2B4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-EIF2B4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF2B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the Central region of human EIF2B4.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EIF2B4 Antibody (Center K161) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow).)

Western Blot (WB)

(EIF2B4 Antibody (Center K161) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the EIF2B4 antibody detected the EIF2B4 protein (arrow).)

Immunohistochemistry (IHC)

(EIF2B4 Antibody (Center K161) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2B4 Antibody (Center K161) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-EIF2B4 antibody

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-EIF2B4 antibody

Antibodies; Translation Initiation Factors

References

Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010): Wu, Y., et al. J. Hum. Genet. 54(2):74-77(2009). Horzinski, L., et al. PLoS ONE 4 (12), E8318 (2009): Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008). Wong, S.S., et al. J. Child Neurol. 23(6):710-714(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 78000158
• NCBI GeneID: 8890
• NCBI Accession #: NP_001029288
• NCBI GenBank Nucleotide #: NM_001034116
• UniProt Accession #: Q9UI10; Q53RY7; Q5BJF4; Q9BUV9; Q9UBG4; Q9UIQ9; Q9UJ95
• Molecular Weight: 57,458 Da
• NCBI Official Full Name: translation initiation factor eIF-2B subunit delta isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
• NCBI Official Symbol: EIF2B4
• NCBI Official Synonym Symbols: EIF2B; EIF-2B; EIF2Bdelta
• NCBI Protein Information: translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; eukaryotic translation initiation factor 2B subunit 4 delta; translation initiation factor eIF-2b delta subunit
• UniProt Protein Name: Translation initiation factor eIF-2B subunit delta
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF2B4
• UniProt Synonym Gene Names: EIF2BD
• UniProt Entry Name: EI2BD_HUMAN

Uniprot Description

eIF2B-delta: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B4 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B alpha/beta/delta subunits family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation initiation; Motility/polarity/chemotaxis; Translation

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; cytosol

Molecular Function: protein binding; translation initiation factor binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity; S-methyl-5-thioribose-1-phosphate isomerase activity

Biological Process: myelination; response to peptide hormone stimulus; negative regulation of translational initiation; translation; cellular response to stimulus; regulation of translation; cellular protein metabolic process; ovarian follicle development; methionine salvage; response to heat; response to glucose stimulus; translational initiation; gene expression; negative regulation of translation initiation in response to stress; oligodendrocyte development; positive regulation of GTPase activity; regulation of translational initiation

Disease: Leukoencephalopathy With Vanishing White Matter