Rabbit anti-Human EIF2AK4 Polyclonal Antibody | anti-EIF2AK4 antibody

EIF2AK4 (GCN2, KIAA1338, GCN2-like protein, Eukaryotic translation initiation factor 2-alpha kinase 4) (HRP)

Cat. Num. AAA6404351

• Gene Names: EIF2AK4; GCN2; PVOD2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: EIF2AK4; Polyclonal Antibody; EIF2AK4 (GCN2; KIAA1338; GCN2-like protein; Eukaryotic translation initiation factor 2-alpha kinase 4) (HRP); anti-EIF2AK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human h.EIF2AK4. No crossreactivity with other proteins.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EIF2AK4 antibody: Western Blot (WB)
• Application Notes: WB: 0.1-0.5ug/ml. The detection limit for EIF2AK4 is ~0.5ng/lane under reducing conditions.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding aa870-888, ATDHLAFSADSKQDDQTGD in the middle region of human EIF2AK4 (Q9P2K8)
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-EIF2AK4 antibody

Antibodies; Translation Initiation Factors

References

1. Berlanga, J. J., Santoyo, J., de Haro, C. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2-alpha kinase. Europ. J. Biochem. 265: 754-762, 1999. 2. Costa-Mattioli, M., Gobert, D., Harding, H., Herdy, B., Azzi, M., Bruno, M., Bidinosti, M., Ben Mamou, C., Marcinkiewicz, E., Yoshida, M., Imataka, H., Cuello, A. C., Seidah, N., Sossin, W., Lacaille, J.-C., Ron, D., Nader, K., Sonenberg, N. Translational control of hippocampal synaptic plasticity and memory by the elF2-alpha kinase GCN2. Nature 436: 1166-1170, 2005. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/28/2005.

NCBI and Uniprot Product Information

• NCBI GI #: 296439368
• NCBI GeneID: 440275
• UniProt Accession #: Q9P2K8; Q69YL7; Q6DC97; Q96GN6; Q9H5K1; Q9NSQ3; Q9NSZ5; Q9UJ56; C9JEC4
• NCBI Official Full Name: Eukaryotic translation initiation factor 2-alpha kinase 4
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 alpha kinase 4
• NCBI Official Symbol: EIF2AK4
• NCBI Official Synonym Symbols: GCN2; PVOD2
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; GCN2 eIF2alpha kinase; general control nonderepressible 2
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 4
• Protein Family: eIF-2-alpha kinase
• UniProt Gene Name: EIF2AK4
• UniProt Synonym Gene Names: GCN2; KIAA1338
• UniProt Entry Name: E2AK4_HUMAN

NCBI Description

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]

Uniprot Description

GCN2: a kinase of the PEK family. Phosphorylates and inhibits eukaryotic initiation factor (eIF)-2 alpha, providing an important protective mechanism during various cellular stress conditions including endoplasmic reticulum (ER) stress.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Translation; Other group; PEK family; GCN2 subfamily

Chromosomal Location of Human Ortholog: 15q15.1

Molecular Function: protein serine/threonine kinase activity; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding

Biological Process: unfolded protein response; negative regulation of translation; cellular response to starvation; protein amino acid phosphorylation; regulation of translational initiation

Disease: Pulmonary Venoocclusive Disease 2, Autosomal Recessive