Rabbit anti-Human G6PC Polyclonal Antibody | anti-G6PC antibody

G6PC, ID (G6PC, G6PT, Glucose-6-phosphatase, Glucose-6-phosphatase alpha) (AP)

Cat. Num. AAA6300653

• Gene Names: G6PC; G6PT; GSD1; G6PC1; GSD1a
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: G6PC; Polyclonal Antibody; ID (G6PC; G6PT; Glucose-6-phosphatase; Glucose-6-phosphatase alpha) (AP); Glucose-6-phosphatase alpha; anti-G6PC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human G6PC.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-G6PC antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within aa123-149 in the central region of human G6PC.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) usingMBS6007920. G6PC (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6007920 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007920 for immunohistochemistry.)

Product Categories/Family for anti-G6PC antibody

Antibodies; Enzymes; Phosphatase

References

1. Tu, E., et al. Hum. Pathol. 41(3):392-400(2010). 2. Samuel, V.T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):12121-12126(2009). 3. Hu, C., et al. Diabetologia 52(3):451-456(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 206729864
• NCBI GeneID: 2538
• UniProt Accession #: P35575; A1L4C0; B4E1C3; K7EL82
• Molecular Weight: 40,484 Da
• NCBI Official Full Name: Glucose-6-phosphatase
• NCBI Official Synonym Full Names: glucose-6-phosphatase, catalytic subunit
• NCBI Official Symbol: G6PC
• NCBI Official Synonym Symbols: G6PT; GSD1; G6PC1; GSD1a
• NCBI Protein Information: glucose-6-phosphatase; G6Pase; G-6-Pase; G6Pase-alpha; glucose-6-phosphatase alpha
• UniProt Protein Name: Glucose-6-phosphatase
• Protein Family: Glucose-6-phosphatase
• UniProt Gene Name: G6PC
• UniProt Synonym Gene Names: G6PT; G-6-Pase; G6Pase
• UniProt Entry Name: G6PC_HUMAN

NCBI Description

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

Uniprot Description

G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family.

Protein type: EC 3.1.3.9; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Endoplasmic reticulum; Carbohydrate Metabolism - starch and sucrose; Transporter; Transporter, SLC family; Membrane protein, multi-pass; Phosphatase (non-protein); Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: glucose-6-phosphatase activity; phosphotransferase activity, alcohol group as acceptor; phosphate binding

Biological Process: response to food; glycogen metabolic process; steroid metabolic process; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; urate metabolic process; triacylglycerol metabolic process; regulation of gene expression; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; transmembrane transport

Disease: Glycogen Storage Disease Ia