Western Blot (WB)
(Western Blot (WB) analysis of EIF1 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate)
Rabbit anti-Human, Mouse EIF1 Polyclonal Antibody | anti-EIF1 antibody
EIF1 Polyclonal Antibody
Gene Names
EIF1; A121; ISO1; SUI1; EIF-1; EIF1A
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
EIF1; Polyclonal Antibody; EIF1 Polyclonal Antibody; Eukaryotic translation initiation factor 1; eIF1; A121; Protein translation factor SUI1 homolog Sui1iso1; SUI1; anti-EIF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
EIF1 polyclonal antibody detects endogenous levels of EIF1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
113
Applicable Applications for anti-EIF1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human EIF1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of EIF1 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of EIF1 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate)
Related Product Information for anti-EIF1 antibody
In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 13kDa
NCBI Official Full Name
eukaryotic translation initiation factor 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 1
NCBI Official Symbol
EIF1
NCBI Official Synonym Symbols
A121; ISO1; SUI1; EIF-1; EIF1A
NCBI Protein Information
eukaryotic translation initiation factor 1
UniProt Protein Name
Eukaryotic translation initiation factor 1
Protein Family
UniProt Gene Name
EIF1
UniProt Synonym Gene Names
SUI1; eIF1
UniProt Entry Name
EIF1_HUMAN
Uniprot Description
EIF1: a translation initiation factor. Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
Protein type: Translation; Translation initiation
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: cytoplasm; nucleus
Molecular Function: translation factor activity, nucleic acid binding; translation initiation factor activity
Biological Process: dosage compensation, by inactivation of X chromosome; translational initiation; response to stress; regulation of translational initiation
Research Articles on EIF1
Similar Products
Product Notes
The EIF1 eif1 (Catalog #AAA3006822) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EIF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the EIF1 eif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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