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Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, Mouse brain tissue, Primary antibody: 160293(EGR4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Rabbit anti-Human, Mouse EGR4 Polyclonal Antibody | anti-EGR4 antibody

EGR4 Rabbit pAb

Gene Names
EGR4; AT133; NGFIC; NGFI-C; PAT133
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
EGR4; Polyclonal Antibody; EGR4 Rabbit pAb; early growth response 4; NGFIC; NGFI-C; PAT133; anti-EGR4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-EGR4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC: 1:25-1:100
Immunogen
Synthetic peptide of human EGR4
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, Mouse brain tissue, Primary antibody: 160293(EGR4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human fetal brain tissue, Mouse brain tissue, Primary antibody: 160293(EGR4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 160293(EGR4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 160293(EGR4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 160293(EGR4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 160293(EGR4 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-EGR4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51kDa
NCBI Official Full Name
early growth response protein 4
NCBI Official Synonym Full Names
early growth response 4
NCBI Official Symbol
EGR4
NCBI Official Synonym Symbols
AT133; NGFIC; NGFI-C; PAT133
NCBI Protein Information
early growth response protein 4
UniProt Protein Name
Early growth response protein 4
UniProt Gene Name
EGR4
UniProt Synonym Gene Names
EGR-4
UniProt Entry Name
EGR4_HUMAN

Uniprot Description

EGR4: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of apoptosis

Research Articles on EGR4

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Product Notes

The EGR4 egr4 (Catalog #AAA4754671) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGR4 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EGR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC: 1:25-1:100. Researchers should empirically determine the suitability of the EGR4 egr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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