ASNA1 recombinant protein

ASNA1, 1-348aa, Human, His tag, E Coli

Cat. Num. AAA204219

• Gene Names: ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ASNA1; 1-348aa; Human; His tag; E Coli; ATPase ASNA1; ARSA-I; ARSA1; ASNA-I; GET3; hASNA-I; TRC40; ASNA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAGVAG WGVEAEEFED APDVEPLEPT LSNIIEQRSL KWIFVGGKGG VGKTTCSCSL AVQLSKGRES VLIISTDPAH NISDAFDQKF SKVPTKVKGY DNLFAMEIDP SLGVAELPDE FFEEDNMLSM GKKMMQEAMS AFPGIDEAMS YAEVMRLVKG MNFSVVVFDT APTGHTLRLL NFPTIVERGL GRLMQIKNQI SPFISQMCNM LGLGDMNADQ LASKLEETLP VIRSVSEQFK DPEQTTFICV CIAEFLSLYE TERLIQELAK CKIDTHNIIV NQLVFPDPEK PCKMCEARHK IQAKYLDQME DLYEDFHIVK LPLLPHEVRG ADKVNTFSAL LLEPYKPPSA Q
• Sequence Length: 348

• Applicable Applications for ASNA1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ASNA1 recombinant protein

ASNA1, also as known as ARSA and TRC40, belongs to the arsA ATPase family. ASNA1 is the human homolog of the bacterial arsA gene. In E Coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials. ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recombinant human ASNA1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ASNA1 recombinant protein

Metabolism

References

Stefanovic S., et al. (2007) Cell 128:1147-1159; Favaloro V., et al. (2008) J. Cell Sci. 121:1832-1840

NCBI and Uniprot Product Information

• NCBI GI #: 50428938
• NCBI GeneID: 439
• NCBI Accession #: NP_004308
• NCBI GenBank Nucleotide #: NM_004317.3
• UniProt Accession #: O43681; Q53FC6; Q92849; A6NHP8; A8K740
• Molecular Weight: 41.2kDa (371aa) confirmed by MALDI-TOF
• NCBI Official Full Name: ATPase ASNA1
• NCBI Official Synonym Full Names: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• NCBI Official Symbol: ASNA1
• NCBI Official Synonym Symbols: GET3; ARSA1; TRC40; ARSA-I; ASNA-I
• NCBI Protein Information: ATPase ASNA1
• UniProt Protein Name: ATPase ASNA1
• Protein Family: ATPase
• UniProt Gene Name: ASNA1

NCBI Description

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]

Uniprot Description

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (). May be involved in insulin signaling.