Rabbit anti-Human, Mouse EGF Polyclonal Antibody | anti-EGF antibody

EGF, ID (EGF, Pro-epidermal growth factor, Epidermal growth factor, Urogastrone) (MaxLight 405)

Cat. Num. AAA6294659

• Gene Names: EGF; URG; HOMG4
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EGF; Polyclonal Antibody; ID (EGF; Pro-epidermal growth factor; Epidermal growth factor; Urogastrone) (MaxLight 405); Urogastrone; anti-EGF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EGF. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-EGF antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 690-720 in the central region of human EGF, UniProt Accession #P01133; NP_001954.2.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in mouse stomach tissue lysates (35ug/lane) using 034958. This demonstrates 034958 detected the EGF protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using 034958 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 034958 for immunohistochemistry.)

Related Product Information for anti-EGF antibody

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-EGF antibody

Antibodies; Growth Factors; Cytokines

References

de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010). Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010). Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010). Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992) Hernandez-Sotomayor, S.M., et al. J. Membr. Biol. 128(2):81-89(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 166362728
• NCBI GeneID: 1950
• NCBI Accession #: NP_001954.2
• NCBI GenBank Nucleotide #: NM_001963.4
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 129,746 Da
• NCBI Official Full Name: pro-epidermal growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]