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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of HLA-DR in human peripheral blood with anti-HLA-DR (L243) Pacific Orange™.)

Mouse HLA-DR Monoclonal Antibody | anti-HLA-DR antibody

Anti-HLA-DR Pacific Orange

Reactivity
Dog, Non-human primates, Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with Pacific Orange under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
HLA-DR; Monoclonal Antibody; Anti-HLA-DR Pacific Orange; anti-HLA-DR antibody
Ordering
Host
Mouse
Reactivity
Dog, Non-human primates, Human
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
L243
Specificity
The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.
Purity/Purification
The purified antibody is conjugated with Pacific Orange under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Pacific Orange. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Applicable Applications for anti-HLA-DR antibody
Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Immunogen
Human B lymphocytes
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of HLA-DR in human peripheral blood with anti-HLA-DR (L243) Pacific Orange™.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of HLA-DR in human peripheral blood with anti-HLA-DR (L243) Pacific Orange™.)
Related Product Information for anti-HLA-DR antibody
HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36kDa alpha chain and 27kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
References
*Coral S, Pucillo C, Leonardi A, Fonsatti E, Altomonte M, Maio M: Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation. Cell Growth Differ. 1997 May;8(5):581-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/9149909,*Muczynski KA, Ekle DM, Coder DM, Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 2003 May;14(5):1336-48., URL: http://www.ncbi.nlm.nih.gov/pubmed/12707403,*Cantin R, Martin G, Tremblay MJ: A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J Gen Virol. 2001 Dec;82(Pt 12):2979-87., URL: http://www.ncbi.nlm.nih.gov/pubmed/11714974,*Swiatek-de Lange M, Rist W, Stahl HF, Weith A, Lenter MC: Comment on "MHC class II expression identifies Functional Application (FUNC)tionally distinct human regulatory T cells". J Immunol. 2008 Mar 15;180(6):3625; author reply 3626., URL: http://www.ncbi.nlm.nih.gov/pubmedterm=comment%20,*De Gassart A, Camosseto V, Thibodeau J, Ceppi M, Catalan N, Pierre P, Gatti E: MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3491-6., URL: http://www.ncbi.nlm.nih.gov/pubmedterm=gassart%20,*Ivanov A, Beers SA, Walshe CA, Honeychurch J, Alduaij W, Cox KL, Potter KN, Murray S, Chan CH, Klymenko T, Erenpreisa J, Glennie MJ, Illidge TM, Cragg MS: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug;119(8):2143-59., URL: http://www.ncbi.nlm.nih.gov/pubmed/19620786,*Kalka-Moll WM, Tzianabos AO, Bryant PW, Niemeyer M, Ploegh HL, Kasper DL: Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions. J Immunol. 2002 Dec 1;169(11):6149-53., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=tzianabos,*Bouillon M, El Fakhry Y, Girouard J, Khalil H, Thibodeau J, Mourad W: Lipid raft-dependent and -independent signaling through HLA-DR molecules. J Biol Chem. 2003 Feb 28;278(9):7099-107., URL: http://www.ncbi.nlm.nih.gov/pubmed/12499388

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Product Notes

The HLA-DR (Catalog #AAA1802212) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HLA-DR Pacific Orange reacts with Dog, Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-DR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Researchers should empirically determine the suitability of the HLA-DR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-DR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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