Rat Cysteinyl Leukotriene Receptor 2 (CYSLTR2) ELISA Kit | CYSLTR2 elisa kit

Rat Cysteinyl Leukotriene Receptor 2 (CYSLTR2) ELISA Kit

Cat. Num. AAA2019604

• Gene Names: CYSLTR2; HG57; CYSLT2; GPCR21; HPN321; CYSLT2R; KPG_011; hGPCR21; PSEC0146
• Reactivity: Rat
• Synonyms: Cysteinyl Leukotriene Receptor 2 (CYSLTR2); Rat Cysteinyl Leukotriene Receptor 2 (CYSLTR2) ELISA Kit; CYSLT2; GPCR; CYSLT2R; HG57; HPN321; HGPCR21; G-protein coupled receptor GPCR21; G-protein coupled receptor HG57; CYSLTR2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteinyl Leukotriene Receptor 2 (CYSLTR2).; No significant cross-reactivity or interference between Cysteinyl Leukotriene Receptor 2 (CYSLTR2) and analogues was observed.
• Sequence Length: 346

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.108ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 2 (CYSLTR2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 2 (CYSLTR2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYSLTR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 2 (CYSLTR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteinyl Leukotriene Receptor 2 (CYSLTR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CYSLTR2 elisa kit

Signal transduction; Infection immunity; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 9966851
• NCBI GeneID: 57105
• NCBI Accession #: NP_065110.1
• NCBI GenBank Nucleotide #: NM_020377.3
• UniProt Accession #: Q9NS75; Q9HCQ2
• Molecular Weight: 39,635 Da
• NCBI Official Full Name: cysteinyl leukotriene receptor 2
• NCBI Official Synonym Full Names: cysteinyl leukotriene receptor 2
• NCBI Official Symbol: CYSLTR2
• NCBI Official Synonym Symbols: HG57; CYSLT2; GPCR21; HPN321; CYSLT2R; KPG_011; hGPCR21; PSEC0146
• NCBI Protein Information: cysteinyl leukotriene receptor 2
• UniProt Protein Name: Cysteinyl leukotriene receptor 2
• Protein Family: Cysteinyl leukotriene receptor
• UniProt Gene Name: CYSLTR2
• UniProt Synonym Gene Names: CYSLT2; CYSLT2R; CysLTR2; hGPCR21
• UniProt Entry Name: CLTR2_HUMAN

NCBI Description

The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR1. This encoded receptor is a member of the superfamily of G protein-coupled receptors. It seems to play a major role in endocrine and cardiovascular systems. [provided by RefSeq, Jul 2008]

Uniprot Description

CYSLTR2: Receptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol- calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4 >> LTE4. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; cysteinyl leukotriene receptor activity; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; immune response