Rabbit anti-Human EGF EGF Polyclonal Antibody | anti-EGF antibody

EGF antibody

Cat. Num. AAA534226

• Gene Names: EGF; URG; HOMG4
• Reactivity: Human EGF
• Applications: ELISA, Western Blot
• Synonyms: EGF; Polyclonal Antibody; EGF antibody; Polyclonal EGF; Anti-EGF; Epidermal Growth FactorUrogastrone; URG; anti-EGF antibody

• Host: Rabbit
• Reactivity: Human EGF
• Clonality: Polyclonal
• Specificity: Human EGF
• Form/Format: Lyophilized from PBS, pH 7.2. Reconstitute in sterile water at a concentration of >0.2 mg/ml.
• Sequence Length: 1207

• Applicable Applications for anti-EGF antibody: ELISA (EIA), Inhibition, Western Blot (WB)
• Application Notes: ELISA: 0.5-2 ug/ml
• Biological Significance: EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF alpha and VGF (vaccinia virus growth factor).
• Immunogen: EGF antibody was raised in rabbit using highly pure recombinant human EGF as the immunogen.
• Preparation and Storage: Store at -20 degree C until reconstitution. Following reconstitution product may be stored at 4 degree C in the short term. For long term storage aliquot and freeze at -20 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-EGF antibody

Affinity purified Rabbit polyclonal EGF antibody

Product Categories/Family for anti-EGF antibody

Cytokines & Growth Factors; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 166362728
• NCBI GeneID: 1950
• NCBI Accession #: NP_001954
• NCBI GenBank Nucleotide #: NM_001963.4
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 6.2 kDa (predicted detection band MW)
• NCBI Official Full Name: pro-epidermal growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase; Hormone

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; extracellular region; integral to membrane

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase activation (dimerization); fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; innate immune response; negative regulation of secretion; angiogenesis; blood coagulation; DNA replication; positive regulation of phosphorylation; positive regulation of DNA binding; positive regulation of granule cell precursor proliferation; STAT protein nuclear translocation

Disease: Hypomagnesemia 4, Renal