EGF active protein

Human EGF

Cat. Num. AAA692154

• Gene Names: EGF; URG; HOMG4
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: EGF; Human EGF; Recombinant Human EGF; Epidermal Growth Factor; Urogastrone; URG; EGF active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: MNSDSECPLS
• Sequence Length: 1166

• N Terminal Sequence: MNSDSECPLS
• Buffer: PBS
• Preparation and Storage: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degree C. Reconstituted EGF should be stored in working aliquots at -20 degree C.

Testing Data

Testing Data #2

Testing Data #3

Related Product Information for EGF active protein

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGFalpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin-binding EGF-like growth factor (HBEGF), epigen, and the neuregulins (NRG) 1 through 6. Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intra-molecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis. The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region. Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members. EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

Product Categories/Family for EGF active protein

Cytokines & Growth Factors

References

1. Harris RC et al, Exp Cell Res 284:2, 2003 2. Carpenter G and Cohen S, J Biol Chem 265:7709, 1990 3. Bell GI et al, Nucl Acids Res 14:8427, 1986 4. Carpenter G and Zendegui JG, Exp Cell Res 164:1, 1986 5. Jorissen RN et al, Exp Cell Res 284:31, 2003 6. Gamett DC et al, J Biol Chem 272:12052, 1997 7. Qian X et al, Proc Natl Acad Sci 91:1500, 1994 8. Qian X et al, J Biol Chem 274:574, 1999

NCBI and Uniprot Product Information

• NCBI GI #: 296011013
• NCBI GeneID: 1950
• NCBI Accession #: NP_001954.2
• NCBI GenBank Nucleotide #: NM_001178131
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 6.35 kDa
• NCBI Official Full Name: pro-epidermal growth factor isoform 2 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Ref.10

Subunit structure: Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF2

By similarity. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD). Ref.11

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed in kidney, salivary gland, cerebrum and prostate. Ref.10

Post-translational modification: O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). Ref.8

Involvement in disease: Hypomagnesemia 4 (HOMG4) [MIM:611718]: A disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to moderate psychomotor retardation, and brisk tendon reflexes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Contains 9 EGF-like domains.Contains 9 LDL-receptor class B repeats.

Sequence caution: The sequence AAR84237.1 differs from that shown. Reason: Erroneous gene model prediction.