Goat EEA1 Polyclonal Antibody | anti-EEA1 antibody

EEA1 Polyclonal Antibody

Cat. Num. AAA448002

• Gene Names: EEA1; MST105; ZFYVE2; MSTP105
• Reactivity: Human, mouse and rat proteins.
• Applications: Immunofluorescence, Western Blot
• Synonyms: EEA1; Polyclonal Antibody; EEA1 Polyclonal Antibody; Anti-EEA1; early endosome antigen 1; 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody; anti-EEA1 antibody

• Host: Goat
• Reactivity: Human, mouse and rat proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.
• Form/Format: Polyclonal antibody supplied as a 200 ul(3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Sequence Length: 1411

• Applicable Applications for anti-EEA1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: 1:250-1:1,000; Immunofluorescence: 1:25-1:250
• Immunogen: Purified recombinant peptide within residues 1230 aa to the C-terminus of human EEA1 produced in E. coli.
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Store at 2-8°C for up to one month.; Store at -20°C for long term.; Avoid freeze/thaw cycles.

Reactivity Chart

Testing Data

Related Product Information for anti-EEA1 antibody

Early Endosome Antigen 1

Goat polyclonal antibody to EEA1 - early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.

NCBI and Uniprot Product Information

• NCBI GI #: 55770888
• NCBI GeneID: 8411
• NCBI Accession #: NP_003557.2
• NCBI GenBank Nucleotide #: NM_003566.3
• UniProt Accession #: Q15075; Q14221
• NCBI Official Full Name: early endosome antigen 1
• NCBI Official Synonym Full Names: early endosome antigen 1
• NCBI Official Symbol: EEA1
• NCBI Official Synonym Symbols: MST105; ZFYVE2; MSTP105
• NCBI Protein Information: early endosome antigen 1; early endosome antigen 1, 162kD; endosome-associated protein p162; early endosome-associated protein; zinc finger FYVE domain-containing protein 2
• UniProt Protein Name: Early endosome antigen 1
• Protein Family: Early endosome antigen
• UniProt Gene Name: EEA1
• UniProt Synonym Gene Names: ZFYVE2
• UniProt Entry Name: EEA1_HUMAN

Uniprot Description

Function: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Subunit structure: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with RAB31. Interacts with ERBB2. May interact with PLEKHF2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.17 Ref.18

Subcellular location: Cytoplasm. Early endosome membrane; Peripheral membrane protein Ref.1 Ref.7 Ref.9 Ref.13 Ref.17.

Domain: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions. Ref.14

Miscellaneous: Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.

Sequence similarities: Contains 1 C2H2-type zinc finger.Contains 1 FYVE-type zinc finger.