Western Blot (WB)
(Anti-EEA1 Antibodyat 1:1000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 162 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit EEA1 Polyclonal Antibody | anti-EEA1 antibody
EEA1 Antibody
Western Blot (WB)
(Anti-EEA1 Antibodyat 1:1000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 162 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-EEA1 Antibodyat 1:1000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 162 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Seelig H.P.,et al.Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
Scherer S.E.,et al.Nature 440:346-351(2006).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Simonsen A.,et al.Nature 394:494-498(1998).
NCBI and Uniprot Product Information
Uniprot Description
EEA1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with ERBB2.
Protein type: C2H2-type zinc finger protein; Vesicle
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: extrinsic to plasma membrane; recycling endosome; membrane; early endosome membrane; cytoplasm; early endosome; cytoplasmic vesicle; cytosol; serine-pyruvate aminotransferase, type 2B complex
Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; zinc ion binding; GTP-dependent protein binding; phosphatidylinositol binding
Biological Process: vesicle fusion; early endosome to late endosome transport; endocytosis; synaptic vesicle to endosome fusion
Research Articles on EEA1
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Product Notes
The EEA1 eea1 (Catalog #AAA9211566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 201-260. The EEA1 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~ 1:1000. Researchers should empirically determine the suitability of the EEA1 eea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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