Western Blot (WB)
(Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).)
Mouse Early Endosome Antigen 1 (EEA1) Monoclonal Antibody | anti-EEA1 antibody
Early Endosome Antigen 1 (EEA1) Antibody
Gene Names
EEA1; MST105; ZFYVE2; MSTP105
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Protein A chromatography
Synonyms
Early Endosome Antigen 1 (EEA1); Monoclonal Antibody; Early Endosome Antigen 1 (EEA1) Antibody; anti-EEA1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M347
Specificity
This antibody detects a 180kDa* protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.
Purity/Purification
Protein A chromatography
Form/Format
Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-EEA1 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:50
WB: 1:1000
ICC: 1:50
WB: 1:1000
Immunogen
Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).)
Western Blot (WB)
(Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (EM3471). The antibody was detected using goat anti-mouse DyLight 488.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (EM3471). The antibody was detected using goat anti-mouse DyLight 488.)
Related Product Information for anti-EEA1 antibody
Early endosomes are cellular compartments that receive endocytosed materials and sort them for vesicular transport to late endosomes and lysosomes, as well as for recycling material to the plasma membrane. Early endosome antigen 1 (EEA1) is an early endosomal protein that contains an N-terminal zinc finger motif, a cys-rich C-terminal metal-binding finger, and multiple sites for N-glycosylation, phosphorylation, and N-myristoylation. Expression of EEA1 mRNA is observed in skeletal muscle, heart, brain, lung, liver, and pancreas. Immunoblot analysis shows that EEA1 is a 180-kDa protein localized in membrane and cytosolic fractions. Immunofluorescence microscopy shows that EEA1 colocalizes with transferrin and with RAB5 in early endosomes, but not with RAB7 in late endosomes.
References
Dumas, J.J. et al. (2001) Mol. Cell 8:947.
Simonsen, A. et al. (1998) Nature 394:494.
Mu, F.T. et al. (1995) J. Biol. Chem. 270:13503.
Simonsen, A. et al. (1998) Nature 394:494.
Mu, F.T. et al. (1995) J. Biol. Chem. 270:13503.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
162,466 Da
NCBI Official Full Name
Homo sapiens early endosome antigen 1 (EEA1), mRNA
NCBI Official Synonym Full Names
early endosome antigen 1
NCBI Official Symbol
EEA1
NCBI Official Synonym Symbols
MST105; ZFYVE2; MSTP105
NCBI Protein Information
early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2
UniProt Protein Name
Early endosome antigen 1
Protein Family
UniProt Gene Name
EEA1
UniProt Synonym Gene Names
ZFYVE2
UniProt Entry Name
EEA1_HUMAN
Research Articles on EEA1
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Product Notes
The EEA1 eea1 (Catalog #AAA474329) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Early Endosome Antigen 1 (EEA1) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Early Endosome Antigen 1 (EEA1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:50 WB: 1:1000. Researchers should empirically determine the suitability of the EEA1 eea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Early Endosome Antigen 1 (EEA1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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