Rabbit anti-Human EDN2 Polyclonal Antibody | anti-EDN2 antibody

EDN2, CT (EDN2, Endothelin-2, Preproendothelin-2) (FITC)

Cat. Num. AAA6294404

• Gene Names: EDN2; ET2; ET-2; PPET2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EDN2; Polyclonal Antibody; CT (EDN2; Endothelin-2; Preproendothelin-2) (FITC); Preproendothelin-2; anti-EDN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EDN2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-136 amino acids from the C-terminal region of human EDN2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EDN2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EDN2 antibody detected the EDN2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of EDN2 (arrow) using rabbit polyclonal EDN2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EDN2 gene.)

Related Product Information for anti-EDN2 antibody

This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. [provided by RefSeq].

Product Categories/Family for anti-EDN2 antibody

Antibodies; Endotoxin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Darrah, R., et al. Physiol. Genomics 41(1):71-77(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Menon, R., et al. Reprod. Biol. Endocrinol. 7, 62 (2009): Wiesmann, F., et al. Breast Cancer Res. 11 (3), R34 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503463
• NCBI GeneID: 1907
• NCBI Accession #: NP_001947.1
• NCBI GenBank Nucleotide #: NM_001956.4
• UniProt Accession #: P20800; Q5T1R3
• Molecular Weight: 19,960 Da
• NCBI Official Full Name: endothelin-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: endothelin 2
• NCBI Official Symbol: EDN2
• NCBI Official Synonym Symbols: ET2; ET-2; PPET2
• NCBI Protein Information: endothelin-2
• UniProt Protein Name: Endothelin-2
• Protein Family: Endothelin
• UniProt Gene Name: EDN2
• UniProt Synonym Gene Names: ET-2; PPET2
• UniProt Entry Name: EDN2_HUMAN

NCBI Description

This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]