Rabbit anti-Human EDN2 Polyclonal Antibody | anti-EDN2 antibody

EDN2, CT (EDN2, Endothelin-2, Preproendothelin-2) (Biotin)

Cat. Num. AAA6294403

• Gene Names: EDN2; ET2; ET-2; PPET2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EDN2; Polyclonal Antibody; CT (EDN2; Endothelin-2; Preproendothelin-2) (Biotin); Preproendothelin-2; anti-EDN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EDN2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-136 amino acids from the C-terminal region of human EDN2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EDN2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EDN2 antibody detected the EDN2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of EDN2 (arrow) using rabbit polyclonal EDN2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EDN2 gene.)

Related Product Information for anti-EDN2 antibody

This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. [provided by RefSeq].

Product Categories/Family for anti-EDN2 antibody

Antibodies; Endotoxin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Darrah, R., et al. Physiol. Genomics 41(1):71-77(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Menon, R., et al. Reprod. Biol. Endocrinol. 7, 62 (2009): Wiesmann, F., et al. Breast Cancer Res. 11 (3), R34 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503463
• NCBI GeneID: 1907
• NCBI Accession #: NP_001947.1
• NCBI GenBank Nucleotide #: NM_001956.4
• UniProt Accession #: P20800; Q5T1R3
• Molecular Weight: 19,960 Da
• NCBI Official Full Name: endothelin-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: endothelin 2
• NCBI Official Symbol: EDN2
• NCBI Official Synonym Symbols: ET2; ET-2; PPET2
• NCBI Protein Information: endothelin-2
• UniProt Protein Name: Endothelin-2
• Protein Family: Endothelin
• UniProt Gene Name: EDN2
• UniProt Synonym Gene Names: ET-2; PPET2
• UniProt Entry Name: EDN2_HUMAN

NCBI Description

This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]