Rabbit anti-Human EDN1 Polyclonal Antibody | anti-EDN1 antibody

EDN1, ID (EDN1, Endothelin-1, Preproendothelin-1, Big endothelin-1) (AP)

Cat. Num. AAA6294390

• Gene Names: EDN1; ET1; PPET1; HDLCQ7
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EDN1; Polyclonal Antibody; ID (EDN1; Endothelin-1; Preproendothelin-1; Big endothelin-1) (AP); Big endothelin-1; anti-EDN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-EDN1 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EDN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from the Central region of human EDN1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EDN1 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EDN1 antibody detected the EDN1 protein (arrow).)

Immunohistochemistry (IHC)

(EDN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZMYND17 EDN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of EDN1 Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(EDN1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS)

(EDN1 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EDN1 antibody

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-EDN1 antibody

Antibodies; Endotoxin

References

Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010). Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010): Feng, J., et al. Circulation 122 (11 SUPPL), S150-S155 (2010): Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010). Kaparianos, A., et al. Eur Rev Med Pharmacol Sci 14(8):705-719(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 154800437
• NCBI GeneID: 1906
• NCBI Accession #: NP_001946.3
• NCBI GenBank Nucleotide #: NM_001955.4
• UniProt Accession #: P05305; Q96DA1; Q6FH53
• Molecular Weight: 24,425 Da
• NCBI Official Full Name: endothelin-1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: endothelin 1
• NCBI Official Symbol: EDN1
• NCBI Official Synonym Symbols: ET1; PPET1; HDLCQ7
• NCBI Protein Information: endothelin-1; OTTHUMP00000016038; preproendothelin-1
• UniProt Protein Name: Endothelin-1
• Protein Family: Endothelin
• UniProt Gene Name: EDN1
• UniProt Synonym Gene Names: PPET1; ET-1
• UniProt Entry Name: EDN1_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding

Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; response to testosterone stimulus; respiratory gaseous exchange; response to amino acid stimulus; peptide hormone secretion; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; positive regulation of cell size; neural crest cell development; G-protein signaling, phospholipase D activating pathway; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; glucose transport; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; calcium-mediated signaling; positive regulation of hormone secretion; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; response to ozone; cartilage development; positive regulation of prostaglandin secretion; maternal process involved in parturition; regulation of sensory perception of pain; positive regulation of cell migration

Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3