Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using EDG6 antibody.)
Rabbit anti-Human, Mouse EDG6 Polyclonal Antibody | anti-EDG6 antibody
EDG6 Antibody
Gene Names
S1PR4; EDG6; LPC1; S1P4; SLP4
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EDG6; Polyclonal Antibody; EDG6 Antibody; Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6; Endothelial differentiation G protein coupled receptor 6; Endothelial differentiation G-protein coupled receptor 6; Endothelial differentiation gene 6; LPC1; Lysophospholipid receptor C1; MGC129298; Putative G protein coupled receptor; S1P receptor 4; S1P receptor Edg-6; S1P receptor Edg6; S1P4; S1PR4; S1PR4_HUMAN; SLP4; Sphingosine 1 phosphate receptor 4; Sphingosine 1 phosphate receptor Edg6; Sphingosine 1-phosphate receptor 4; Sphingosine 1-phosphate receptor Edg-6; anti-EDG6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
EDG6 antibody detects endogenous levels of total EDG6
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
384
Applicable Applications for anti-EDG6 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using EDG6 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using EDG6 antibody.)
Immunofluorescene (IF)
(MBS9606324 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9606324 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-EDG6 antibody
Function: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.
Similarity: Belongs to the G-protein coupled receptor 1 family.
Similarity: Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 40 kD
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
sphingosine 1-phosphate receptor 4
NCBI Official Synonym Full Names
sphingosine-1-phosphate receptor 4
NCBI Official Symbol
S1PR4
NCBI Official Synonym Symbols
EDG6; LPC1; S1P4; SLP4
NCBI Protein Information
sphingosine 1-phosphate receptor 4
UniProt Protein Name
Sphingosine 1-phosphate receptor 4
UniProt Gene Name
S1PR4
UniProt Synonym Gene Names
EDG6; S1P receptor 4; S1P4; S1P receptor Edg-6
NCBI Description
This gene is a member of the endothelial differentiation, G-protein-coupled (EDG)) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands, and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.
Research Articles on EDG6
1. High S1PR4 expression is associated with anti-neutrophil cytoplasmic antibody-associated vasculitis.
Similar Products
Product Notes
The EDG6 s1pr4 (Catalog #AAA9606324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EDG6 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EDG6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the EDG6 s1pr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDG6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
