Rat Heat Shock Protein 60 ELISA Kit | HSP-60 elisa kit

Rat Heat Shock Protein 60 ELISA Kit

Cat. Num. AAA764595

• Gene Names: HSP60; heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
• Reactivity: Rat
• Synonyms: Heat Shock Protein 60; Rat Heat Shock Protein 60 ELISA Kit; HSP-60/HSPD1 (Heat Shock Protein 60)/cpn60/GROEL/SPG13/60 kDa chaperonin/Chaperonin 60/heat shock 60kD protein 1 (chaperonin)/Heat shock protein 60/heat shock protein 65/HLD4/Hsp60/HSP65/HuCHA60/P60 lymphocyte protein/short heat shock protein 60 Hsp60s1/spastic paraplegia 13 (autosomal dominant); HSP-60 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSP-60. No significant cross-reactivity or interference between HSP-60 and analogues was observed.
• Sequence Length: 577

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HSP- 60 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HSP-60 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP-60 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HSP-60 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HSP-60 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HSP-60 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HSP-60 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15229559
• NCBI GeneID: 821983
• NCBI Accession #: NP_189041.1
• NCBI GenBank Nucleotide #: NM_113304.4
• Molecular Weight: 61,281 Da
• NCBI Official Full Name: heat shock protein 60
• NCBI Official Symbol: HSP60
• NCBI Official Synonym Symbols: heat shock protein 60; HEAT SHOCK PROTEIN 60-3B; HSP60; HSP60-3B
• NCBI Protein Information: heat shock protein 60
• UniProt Protein Name: Chaperonin CPN60, mitochondrial
• Protein Family: Heat shock protein
• UniProt Gene Name: CPN60
• UniProt Entry Name: CH60A_ARATH

NCBI Description

mitochondrial chaperonin HSP. assist in rapid assembly of the oligomeric protein structures in the mitochondria.

Uniprot Description

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.