Rabbit ECM1 Polyclonal Antibody | anti-ECM1 antibody

ECM1 (Extracellular Matrix Protein 1, Secretory Component p85) (FITC)

Cat. Num. AAA6414938

• Gene Names: ECM1; URBWD
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ECM1; Polyclonal Antibody; ECM1 (Extracellular Matrix Protein 1; Secretory Component p85) (FITC); anti-ECM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ECM1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ECM1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ECM1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ECM1 antibody.)

Product Categories/Family for anti-ECM1 antibody

Antibodies; Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 322302700
• NCBI GeneID: 1893
• NCBI Accession #: NP_001189787.1
• NCBI GenBank Nucleotide #: NM_001202858.1
• UniProt Accession #: Q16610; O43266; Q5T5G4; Q5T5G5; Q5T5G6; Q8IZ60; A8K8S0; B4DW49; B4DY60
• Molecular Weight: 60,674 Da
• NCBI Official Full Name: extracellular matrix protein 1 isoform 3
• NCBI Official Synonym Full Names: extracellular matrix protein 1
• NCBI Official Symbol: ECM1
• NCBI Official Synonym Symbols: URBWD
• NCBI Protein Information: extracellular matrix protein 1; secretory component p85
• UniProt Protein Name: Extracellular matrix protein 1
• Protein Family: Extracellular matrix protein
• UniProt Gene Name: ECM1
• UniProt Entry Name: ECM1_HUMAN

NCBI Description

This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; enzyme binding; protease binding; interleukin-2 receptor binding; laminin binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of peptidase activity; signal transduction; regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; positive regulation of angiogenesis; biomineral formation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; angiogenesis; regulation of T-helper 2 type immune response; inflammatory response

Disease: Lipoid Proteinosis Of Urbach And Wiethe